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Objective
to review the evidence from studies relating SARS-CoV-2 culture with the results of reverse transcriptase polymerase chain reaction (RT-PCR) and other variables which may influence the interpretation of the test, such as time from symptom onset
Methods
We searched LitCovid, medRxiv, Google Scholar and the WHO Covid-19 database for Covid-19 to 10 September 2020. We included studies attempting to culture or observe SARS-CoV-2 in specimens with RT-PCR positivity. Studies were dual extracted and the data summarised narratively by specimen type. Where necessary we contacted corresponding authors of included papers for additional information. We assessed quality using a modified QUADAS 2 risk of bias tool.
Results
We included 29 studies reporting attempts at culturing, or observing tissue infection by, SARS-CoV-2 in sputum, nasopharyngeal or oropharyngeal, urine, stool, blood and environmental specimens. The quality of the studies was moderate with lack of standardised reporting. The data suggest a relationship between the time from onset of symptom to the timing of the specimen test, cycle threshold (Ct) and symptom severity. Twelve studies reported that Ct values were significantly lower and log copies higher in specimens producing live virus culture. Two studies reported the odds of live virus culture reduced by approximately 33% for every one unit increase in Ct. Six of eight studies reported detectable RNA for longer than 14 days but infectious potential declined after day 8 even among cases with ongoing high viral loads. Four studies reported viral culture from stool specimens.
Conclusion
Complete live viruses are necessary for transmission, not the fragments identified by PCR. Prospective routine testing of reference and culture specimens and their relationship to symptoms, signs and patient co-factors should be used to define the reliability of PCR for assessing infectious potential. Those with high cycle threshold are unlikely to have infectious potential.
A PCR false positive pseudo-epidemic looks just like a real epidemic, but isn’t... We have very strong evidence that the PCR mass testing as currently conducted is completely worthless.
lockdownsceptics.org...
"I think the evidence is unequivocal that we are in a PCR false positive pseudo-epidemic..." (Dr. Michael Yeadon)
COVID Pandemic Is Over, No Need For Vaccines, Says Former Vice President Of Pfizer
That daily picture they show you, with the ‘cases’ climbing up on the right-hand side? Its horribly exaggerated. This test is fatally flawed and MUST immediately be withdrawn and never used again in this setting unless shown to be fixed.
Lies, Damned Lies and Health Statistics – the Deadly Danger of False Positives
I have explained how a hopelessly-performing diagnostic test has been, and continues to be used, not for diagnosis of disease but, it seems, solely to create fear. (Dr. Michael Yeadon)
lockdownsceptics.org...
jaymacabc123 4 hours ago
I keep hearing that the vaccine can potentially change the individuals DNA and the DNA of future generations. Will anyone pls explain - in layman's terms - how this can occur. I always thought that the only way to change DNA was to totally obliterate the bone marrow and have a bone marrow transplant / stem cell transplant (such as is the case for many people who have blood cancer - my husband is an acute meloid leukemia survivor)
Roberto Philippo 4 hours ago
This vaccine wasn't made in a few months, that's impossible, this vaccine is created a long time ago... Given the new architecture, it is a longer-standing resource. To perfect a tool on an RNA basis is an impossible task in this short space of time. This method of vaccine composition has been under research for some time at Darpa and Gavi. This must be a means that has been stored for a long time and we can therefore speak of a premeditation to the inoculation of the population. The appearance of research is already a hoax. There is a conscious structure leading to the administration of the drug and we are threatened with that, given the unpredictability of this vaccine. People should already be resisting these plans because it intends to eliminate large parts of humanity. Anyone who threatens "we the people" must take into account a reaction of the same proportions. Human rights will triumph if it is with our blood.
JohnnyRedsnick 6 hours ago
fake actor doctors. If coronavirus is not real then why are my friends and family falling like flies?
AnitaBee 6 hours ago
Whatever they are falling from is being misattributed to COVID. It doesn't matter what they are sick with. PCR is not a diagnostic test. The whole business is a fraud.
pedronumerouno 7 hours ago
two nhs nurses had reaction to pfiser vax.
it is nothing t do with allergic reactions as they are trying to make out.you are alergic to nuts like boris, your alergic to bee stings. those bring on anaphylactic shock. This is not anaphylactic shock. this a autoimmune response to the viral toxins being injected into the body. the vaccine is full of toxic substances, of which there has not been a trim sheet released, trim sheet is proof of toxic contents. there is n release of insert regarding contents not warnings about side effects. on the 2 dec matt small cock was asked by. minister in the house of commons, " how did you get approval so quickly" he replied, " we changed the law". wake up peeps.
GaryRozar 7 hours ago
WE KNOW NOW THAT THERE ARE OVER 20,000 DOCTORS THAT HAVE SIGNED PAPERS SAYING THAT THE CVD19 IS A SCAM AND NOT TO TAKE THE VACCINE! THIS VIDEO MUST BE DOWN LOADED FOR FUTURE USE...THEY WILL TRY TO CRASH THIS SITE!
brandnewtube.com...
originally posted by: chr0naut
a reply to: Flesh699
U.S. awaits vaccine approval as daily national deaths top 3,000 - Reuters
Apparently, some of the tests were detecting something.
originally posted by: ketsuko
Been saying that for a while now.
PCR can tell you if you have fragments of virus, but that doesn't tell you if you are or ever were actually ill.
originally posted by: Breakthestreak
The tests are a fraud
The fake numbers are a fraud (3000 per day - that’s a lie)
The lockdowns are a fraud
The whole thing is a fraud
And the lowest of IQ’s accept it as ALL as fact
originally posted by: Breakthestreak
a reply to: chr0naut
Take a look at those who rabidly oppose the President , “the left”
Intelligent bunch they are not
You’re easily fooled and easily lead
I wonder why that is.
originally posted by: chr0naut
a reply to: Flesh699
U.S. awaits vaccine approval as daily national deaths top 3,000 - Reuters
Apparently, some of the tests were detecting something.
originally posted by: Flesh699
originally posted by: chr0naut
a reply to: Flesh699
U.S. awaits vaccine approval as daily national deaths top 3,000 - Reuters
Apparently, some of the tests were detecting something.
No. Sorry. I've been in the middle of this since the start and caught covid twice.
Your lungs hurt for two weeks.
You're being played.
And yes, academics are idiots. I previously worked under them. They have zero clue what it's like to work, let alone anything remotely real.
Get off your ass and work. It's actually almost sad I'm forced to get my news from various conspiracy sites because the media is so bad at their job.