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I need some help on the DNA analysis.
Can anyone tell me the following:
1. Re the 15 out of 19 markers. Do we know if any other family member shares all 15 markers - not just in theory but in reality?
2. Do we know whether the analysis could determine if the DNA came from a living or a dead body? If so, do we know which it was?
3. If the 15 out of 19 markers are consistent with it being Madeleine, what are the odds of it being someone else? Are we talking 10 to 1, 100 to 1, 1000 to 1 or more?
4. Do we have a record yet of the initial FSS assessment?
Beachy's reply:
Bomaris, precise answers to some of your questions would be impossible without actually having the results of the DNA testing in front of us, but this is what I can tell you:
For the purposes of this discussion, let us call the sample of Madeleine's DNA that was obtained from her pillow in Rothley or wherever they got it the "known sample." The DNA found in the Scenic and the apartment would have been "questioned samples."
Regardless of the number of markers you are testing, if the DNA in ANY of the markers in a questioned sample does not match the DNA in the known sample, then the questioned sample does not come from the same person who was the donor of the known sample of DNA.
I have not seen anything in writing to date, but I have been assuming that the FSS was able to extract 15 markers from the sample of DNA found in the back of the Renault Scenic, and that the other four markers were so degraded (by heat, sunlight, efforts to clean them up, whatever) that they could not be analysed. If they had 4 out of 19 markers that clearly were NOT a match for Madeleine's DNA, that would have been the end of it, in my opinion; the DNA from the Scenic could not have been hers.
I have read that the Portuguese require 19 markers for a conclusive result on a DNA analysis because under their laws that is how many markers are required for a positive match on a paternity test. This is more markers than I have ever heard of being required anywhere for forensic purposes in a criminal case. In the UK, 10 markers are tested, plus the sex of the donor is determined, and a 10:10 match is considered conclusive. In America, the FBI CODIS database contains 13 markers. Individual states in America are allowed to pass their own laws about how many markers must be tested before evidence can be introduced in court, but most of the 50 states use 13 because there is so much interface with the FBI database. Therefore, in America, a 13:13 match is considered conclusive that the questioned DNA and the known DNA came from the same person. Neverthess, if Portuguese law requires analysis of 19 markers, then that's what must be done. Period.
Regarding your specific questions:
(1) Only identical twins are born with identical DNA, and even in that case, every individual on earth begins to accumulate mutations to his/her DNA that may make it possible to distinguish even between the DNA of identical twins. There is a laboratory in Texas called Orchid Cellmark that claims it already can do this, but so far as I know, this technique has never been used in court.
The DNA of everyone on earth is at least a 99% match. Yep, that's right. The DNA of the most profoundly mentally disabled person who ever lived was a 99% match for Albert Einstein's. The DNA of the poorest beggar on the streets of the poorest city in the world, whoever that unfortunate soul happens to be, is a 99% match for the Queen's. Rather humbling, isn't it? (Note: Studies published in 2001 indicated that the DNA of all human beings was about 99.9% alike. More recent information, obtained from the human genome project, indicates that the accurate figure is probably somewhere in the range of 99 - 99.5%.)
The DNA of siblings is even more alike than that of individuals selected at random, which makes sense, considering that they inherit their DNA from the same two people. Within that 1% or less variation, however, there are literally tens of thousands of different combinations that make the DNA of any one individual unique from that of everyone else, including his/her siblings.
The FBI's CODIS database, which contains the DNA profiles of approximately 6 million convicted criminals, has been extensively studied. No 13:13 match of genetic markers has ever been found except between identical twins. There was a widely reported case several years ago in which a forensics examiner for the state of Arizona in America found a 9:13 match between two unrelated individuals, and there has also been a report of a 10:13 match between two related individuals who were products of an incestuous relationship.
Given the experience with CODIS, I think it is highly, highly unlikely (as in, the odds in favour of it would be one in the tens of millions) that one would find a 15:15 match on genetic markers between two different members of the McCann family.
(2) As I have posted before, DNA cannot be used to determine whether a person was living or dead at the time the sample was taken. A DNA sample taken by swabbing the inside of the cheek of a living person one hour before death and another sample taken from the same person one hour after death would look identical under a microscope. What MIGHT be possible, and it would depend on several different things, including the degree of experience and skill of the forensic examiner and the quantity of DNA available, would be that a forensic examiner asked to attempt to extract DNA from some object that appeared to contain a substance that might be a bodily fluid - a piece of clothing, say, or a piece of carpet from an automobile - MIGHT be able to recognise the type of fluid and therefore tell whether the donor had been alive or dead. There are certain types of fluids - one is an exudate from the lungs that is only seen after death - that might be recognisable as such. In Madeleine's case, however, with so little material available, I am virtually certain that this would not have been possible, i.e., it would not be possible to tell whether the donor of the questioned sample of DNA found in the back of the Scenic was alive or dead at the time the DNA was deposited there. (3) If the forensic technicians were able to extract 15 markers from the material in the Scenic that were a match for the known sample of Madeleine's DNA and the other four markers could not be tested because they were degraded, there would be a high probability mathematically that the questioned sample of DNA came from Madeleine. Just to give you an example, at the time the forensic examiner in Arizona found the 9:13 match on DNA markers, the FBI said that the chances of that happening would be 1 in 113 billion. Well, that obviously isn't right, because there WAS, in fact, a 9:13 match, and there are nowhere near 113 billion people in the world. There is something called the "prosecutor's fallacy," which is an example of mathematical analysis called "binary classification" which shows that even 10:10 or 13:13 DNA matches are subject to error rates much higher than prosecutors sometimes attribute to them. However, whilst saying that the chance of an incorrect finding is 1 in 113 billion is clearly ridiculous, my opinion would be that the chance of two DNA samples belonging to different people if the results of the forensic analysis shows a 15:19 match would be miniscule - at least 1 out of hundreds of thousands, if not millions. It would not, however, be a smoking gun. Any DNA scientist will tell you that DNA is only one piece of the puzzle in any case and should be viewed in the context of all the other evidence. However, if FSS got a 15:19 match between Madeleine's known DNA and the questioned sample from the hire car, and 4 other markers were too degraded to be tested, in my opinion, that would be a powerful piece of circumstantial evidence. But in fact, I don't know exactly what they got. I don't understand what John Lowe is saying. This is the statement from him that I find so troubling: "Let's look at the question that is being asked: 'Is there DNA from Madeleine on the swab?' It would be very simple to say 'yes' simply because of the number of components within the result that are also in her reference sample. What we need to consider, as scientists, is whether the match is genuine - because Madeleine has deposited DNA as a result of being in the car or whether Madeleine merely appears to match the result by chance."
Originally posted by Gordi The Drummer
Hello again Dr!
Just a small point from your last post...
They were smart enough to possibly dispose of a body days before they raised the alarm, feed a story about kidnappers to the British press (who acted completely biased and xenopobic) and tell the police that they left apartment doors OPEN to make checking on their child easier and at the same time easier for an abductor to get in, ie. setting the scene for their version of events.
The last known photo of Maddie, which was later used as one of the press-release photos to publicise her disappearance, was quoted as being taken by the poolside, just 7 hours before her disappearance.
also, other holidaymakers are quoted as having seen Maddie alive and well, on the day of her disappearance, so I'm not sure where the "days before" they raised the alarm point comes in?
cheers
GTD
Originally posted by thedoctorswife
Originally posted by AmberLeaf
Cadever dogs dont lie, they killed her and got away with murder.
I havent believed a word they said since i learned of the cadever dogs findings.
I remember that from a documentary, the dogs were convinced they could smell a dead body, and your right sniffer dogs dont get it wrong, however, it might not necessarily be Madeline, but whoa, what a coincidence if it was someone else eh? i mean, how many random dead bodies have you found in your street? it doesnt happen everyday.
My gut feelings is that Maddie is no longer with us, bless her soul, i just get the feeling that there was an accident.I dont think there was a murder.
Originally posted by kingears
This subject is a tough one.
I myself have a 2 year old daughter. I would NEVER leave her alone in a hotel room, and I'm an unemployed man who has HALF the intelligence her parents (two doctors) are meant to have.
This is what I don't get. They are obviously intelligent human beings, so why would they leave their daughter in a hotel room in Portugal, with her two younger siblings? Something doesn't add up.
I personally believe she was killed, by accident. Whether it was something maddie did whilst she was unsupervised, or they did (just my opinion as there's no proof obviously) but the search for Maddie is a charade to cover their tracks. Like I said they are obviously two intelligent people who could, if needed, partake in a charade of searching for their daughter, whilst covering over any wrong doing they may or may not of done themselves.
I'm not alone in thinking they may (or may not) of killed/covered up their daughters death, this survey says it all ---
www.helium.com...
I hope that someday she is found alive and is reunited with her family, but I fear that this will never happen.
King
Originally posted by kingears
This subject is a tough one.
I myself have a 2 year old daughter. I would NEVER leave her alone in a hotel room, and I'm an unemployed man who has HALF the intelligence her parents (two doctors) are meant to have.
This is what I don't get. They are obviously intelligent human beings, so why would they leave their daughter in a hotel room in Portugal, with her two younger siblings? Something doesn't add up.
I personally believe she was killed, by accident. Whether it was something maddie did whilst she was unsupervised, or they did (just my opinion as there's no proof obviously) but the search for Maddie is a charade to cover their tracks. Like I said they are obviously two intelligent people who could, if needed, partake in a charade of searching for their daughter, whilst covering over any wrong doing they may or may not of done themselves.
I'm not alone in thinking they may (or may not) of killed/covered up their daughters death, this survey says it all ---
www.helium.com...
I hope that someday she is found alive and is reunited with her family, but I fear that this will never happen.
King
Originally posted by old_god
There is a story of some businessman who went out to the site of the disappearance and at considerable cost scanned the grounds for anomalies.
He found one and believed it to grave of Maddie McCann...which seems very likely.
A South African property developer claims Madeleine McCann’s body was buried near the site of her disappearance and that he has found her grave. Stephen Birch says ground radar scans he made with specialist equipment have been passed to Scotland Yard, who are conducting an investigative review of the case.
Originally posted by Staroth
reply to post by ProfessorT
Check this out from May 8. 2012 This witness claims she was with a German family plus 2 other kids as well. This may really be her!
'Groggy' girl seemed alienated from German family she was with at Cabopino campsite near Fuengirola
www.dailymail.co.uk...
Originally posted by Amanda5
reply to post by badkittie748
I would like to know more about the lives of the parents and some further background on where they took their holidays and at what times of the year etcetera. I acknowledge all the brilliant thinking in the posts within this thread but I am returning to my Police experience.edit on 1-9-2012 by Amanda5 because: Spelling
THE REAL VICTIM IS THE MISSING CHILD In a criminal investigation, knowledge of the victim is essential. A physical description is not enough. Her personality, her habits, other interests, her family background and her friendships allow a better understanding of the conditions in which the crime was committed. Knowing about her actions and her movements before her disappearance or her death also helps to determine the motive for the crime. The work is made easier when it's about an adult person with real life experience. When the victim is a child, the information becomes more piecemeal, and it's not easy to define a still evolving personality. All the information about her comes from her parents, her family, their friends, employees, neighbours and sometimes educators. It's not her actions that speak for her, but other people. According to statistics, including Great Britain, parents and close relatives are involved in the majority of cases of missing children. Certainly that does not constitute proof. A common sense rule, however, says doubt their word, without this meaning that they are to be considered as suspects. The information they provide must be cross-checked against other witness statements, in order to evaluate their veracity and credibility. The public in general, deeply touched by the misfortune that has befallen the family - they can all easily imagine the anxiety and pain that a mother or a father must feel in such a situation - take their side right away. The investigator, however, cannot lose sight of his objectives. He has to devote all his efforts to the discovery of the truth in order to bring justice to the only true victim: the child.