I'm replying anonymously since I'm replying from work.
- HIV tests are unreliable and can show a positive result with many other conditions or no conditions whatsoever.
This is true, some tests can give false positives. The way a person is currently diagnosed with HIV. I'd say pregnant women sometimes came up as
positive on our analyses. These tests look for antibodies to the virus that are present in the blood of infected individuals. ELISA, which stands for
enzyme-linked immunosorbent assay, is the primary test used and if it comes up positive, we usually run a Western blot to confirm the presence of HIV.
ELISA is quite sensitive in chronic HIV infection, but because antibodies aren't produced immediately upon infection, a person may test negative
during a few weeks to a few months after being infected. The combination of ELISA and Western Blot used for the diagnosis of HIV is remarkably
accurate, with very low false-positive and -negative rates.
The antibodies assayed by ELISA include but are not limited to those developed against p24 antigen, part of HIV gag which forms part of the capsid,
gp120 which forms the viral envelope and Tat protein which is the transcriptional transactivator.
- there is NO test, to proove the presence of HIV, only supposed HIV antibodies - HIV was never trully isolated (there is a reward to be collected for
anyone who can isolate it, or even proove it already was by showing the documents) Since it wasn't isolated, how can we then test for it, or even
prove it's existance?
We have the viral sequence and so forth... There's have isolated samples from blood, spleen and various brain tissues. It's isolated via density
gradient. This is one part I do not understand of the whole conspiracy. Trust me when I say there is no reward of any kind. Otherwise, let me know
where we can go claim it as I can surely use the money. The NIH is spending a lot of dough on grants dealing with HIV infection. You can isolate the
virus, sequence it, use it to infect cells, run a TCID assay (Infectivity dose), heck even infect another person with the concentrated virus stock you
isolated.
- "virus count" tests are in reality DNA multipliers (PCR) and are not supposed to be used in a quantifying manner (says Kerry Mullins - the
discoverer of PCR, who won the nobel prize for it. He also believes HIV doesn't cause AIDS) Since they multiply the DNA (or DNR in this case) they
multiply it's numbers, and if you then coun't the multiplication you get the result of it, and not the original number
Another way to quantify the virus is through the activity of its reverse transcriptase, the enzyme responsible for viral replication by converting
viral RNA into DNA. After this crucial step, Integrase will add the viral DNA into the cell's own DNA.
Just wanted to clear up on those matters. Now, as to how HIV causes AIDS... Infect a person with the isolated virus, heck a rhesus macaque or a SCID
mouse might work too , and see how long it takes for the CD4+ T cells to be depleted. AIDS stands for Acquired Immune Deficiency Syndrome, meaning
something is causing it. That something in this case is HIV. HIV is able to infect these cells using the CD4 receptor and CXCR4 as a coreceptor. After
replication, CD8+ T cells (cytotoxic) and macrophages recognize the infection inside the CD4 and decide to get rid of them. Most commonly, by
apoptosis or programmed cell death. No CD4 means no antibodies, therefore the immune system goes bye bye and can't get rid of the remaining virus.
This virus can create reservoirs by using our own cells but that's another story.