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Antiproliferative mechanism of a cannabinoid agonist by cell cycle arrest in human gastric cancer cells.
Park JM, Xian XS, Choi MG, Park H, Cho YK, Lee IS, Kim SW, Chung IS.
Source
Division of Gastroenterology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
For gastric cancers, the antineoplastic activity of cannabinoids has been investigated in only a few reports and knowledge regarding the mechanisms involved is limited.
[red]We have reported previously that treatment of gastric cancer cells with a cannabinoid agonist significantly decreased cell proliferation and induced apoptosis. [/red]
Here, we evaluated the effects of cannabinoids on various cellular mediators involved in cell cycle arrest in gastric cancer cells. AGS and MKN-1 cell lines were used as human gastric cancer cells and WIN 55,212-2 as a cannabinoid agonist. Cell cycles were analyzed by flow cytometry and western blotting.
Treatment with WIN 55,212-2 arrested the cell cycle in the G0/G1 phase. WIN 55,212-2 also upregulated phospho-ERK1/2, induced Kip1/p27 and Cip1/WAF1/p21 expression, decreased cyclin D1 and cyclin E expression, decreased Cdk 2, Cdk 4, and Cdk 6 expression levels, and decreased phospho-Rb and E2F-1 expression.
ERK inhibitor decreased the proportion of G0/G1 phase which was induced by WIN 55,212-2.
Inhibition of pAKT led to cell cycle arrest in gastric cancer cells. Cell cycle arrest preceded apoptotic response.
Thus, this cannabinoid agonist can reduce gastric cancer cell proliferation via G1 phase cell cycle arrest, which is mediated via activation of the MAPK pathway and inhibition of pAKT.
Copyright © 2011 Wiley-Liss, Inc.
PMID: 21312237 [PubMed - indexed for MEDLINE]
Originally posted by Turkenstein
Hello everyone. I know there are a lot of sources of information and insight here on ATS. My dad has stomach cancer and I was wanting to here of things that can help. Foods to eat, treatments, comforts, ect..... I really want to help him in anyway I can. I know there are things out there that could help and are not persued because of low profitability and the inability to patent a plant. If you ask me it is truly selfish to not use our resources to find cures instead of just experimenting with peoples lives and seeing what they can take. I'm calling B.S. and saying it is time to start finding cures instead of treatments.
Originally posted by Starwise
reply to post by Turkenstein
My experience is that stomach cancer is deadly, and usually only diagnosed in late stages. It has a poor prognosis. I have only seen surgery to remove parts of the stomach work for a little while, and the quality of life disappears once the patient is subjected to radiation and chemo........I guess prayer never is a bad thing. I do not know of any treatment to actually cure it, only delay the return of it. Blessings to you and your family. I am sorry.