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Quantitative visualization of DNA G-quadruplex structures in human cells
Giulia Biffi, David Tannahill, John McCafferty & Shankar Balasubramanian
Nature Chemistry (2013) | doi:10.1038/nchem.1548
Received 17 October 2012 | Accepted 06 December 2012 | Published online 20 January 2013
Abstract
Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function.
Because they have nothing to do with Rodin's use of the word vortex. If you think you can provide a link, by citing a historical mathematical description of a vortex, and showing how that's related to Rodin's mathematical description of a vortex, that would be on-topic, but since Rodin has no mathematical description of a vortex, that's not possible.
Originally posted by beebs
Your argument is that historical physical theories and physicists that talk about vortices should not be a part of this thread because there is no evidence for Rodin's theory?
What point did I miss?
To me, it seems then that you have missed the point AND have ignored an attempt to increase the level of discussion.
Originally posted by wujotvowujotvowujotvo
Rodin did not claim a material third helix, but an energetic(his definition of that "higher
dimensional Bioaetheric Template
(Morphogenetic Field) defined by
the mathematical number pattern 3,
9; 6; 6, 9, 3") field in the given constrained space of DNA, and labeled this concept as a third helix. Anything to the contrary is misrepresenting Rodin's claim.
Any person with reading comprehension see that critics are saying the same thing as ImaFungi stated
Which version? ImaFungi flip-flopped. First said it sounds like a "spiral of space", then said "guess not". That resolved the discrepancy but you don't seem to be able to keep up.
Originally posted by wujotvowujotvowujotvo
Any person with reading comprehension see that critics are saying the same thing as ImaFungi stated
Originally posted by Arbitrageur
That's not Rodin...did you read what Rodin said? Here's a longer version of the quote (ibid):
Originally posted by ImaFungi
tandem.bu.edu...
it seems hes saying there can be imagined a spiral of space represented by the differences in grooves
en.wikipedia.org/wiki/File:ADN_animation.gif
DNA is not comprised of two helices but from three ...
Do you really think that sounds like a "spiral of space"??
Originally posted by ImaFungi
hmm guess not.. guess it sounds more like a field in a space
Originally posted by Arbitrageur
Which version? ImaFungi flip-flopped. First said it sounds like a "spiral of space", then said "guess not". That resolved the discrepancy but you don't seem to be able to keep up.
Originally posted by wujotvowujotvowujotvo
Any person with reading comprehension see that critics are saying the same thing as ImaFungi stated
Originally posted by Arbitrageur
That's not Rodin...did you read what Rodin said? Here's a longer version of the quote (ibid):
Originally posted by ImaFungi
tandem.bu.edu...
it seems hes saying there can be imagined a spiral of space represented by the differences in grooves
en.wikipedia.org/wiki/File:ADN_animation.gif
DNA is not comprised of two helices but from three ...
Do you really think that sounds like a "spiral of space"??
Originally posted by ImaFungi
hmm guess not.. guess it sounds more like a field in a space
I was giving exact quotes from Rodin.
Yes, that's typical of trolls.
Originally posted by ImaFungi
I was trying to make a snide remark
It seems hes speaking about the space between the two strands, as being a third invisible helix
hmm guess not.. guess it sounds more like a field in a space
Originally posted by wujotvowujotvowujotvo
reply to post by Arbitrageur
As it is an argument now and not a debate for tens of pages, in the last few pages of analysing flow of posts, that was a non-issue.
You were all-inclusive, adding the third helix under Rodin's own physics framework.
Mary Rose didn't presume that you talked about an energetic helix, as you spoke in a non-descriptive generic way.
Originally posted by Arbitrageur
He also says that DNA is a triple helix, whereas all observational evidence points to a double helix. He has no evidence for a triple helix, it seems to be some kind of obsession with the number three that he claims there's a third helix that nobody has ever seen.
Originally posted by Arbitrageur
He also says that DNA is a triple helix, whereas all observational evidence points to a double helix. He has no evidence for a triple helix, it seems to be some kind of obsession with the number three that he claims there's a third helix that nobody has ever seen.
The backbone of DNA’s double spiral helix is comprised of phosphates and phosphates are known to always have a negative electric charge and obviously with any negative electric charge there is always going to be an associated magnetic field.
Originally posted by Mary Rose
reply to post by buddhasystem
Mary is the only person who knows for sure what Mary meant.
Clearly Arbitrageur was insinuating that Rodin thinks we have 3 strands of DNA when the whole wide world knows otherwise. Keyword: "seen":
Originally posted by Arbitrageur
He also says that DNA is a triple helix, whereas all observational evidence points to a double helix. He has no evidence for a triple helix, it seems to be some kind of obsession with the number three that he claims there's a third helix that nobody has ever seen.
So is it now your position that Rodin is contradicting himself, one moment claiming there are three helices and the next claiming there are only two?
Originally posted by Mary Rose
No "triple helix" there.
. . . being invisible and occupying a displacement space called the Major Groove which is located in between the two helical strands.
Originally posted by Mary Rose
reply to post by Arbitrageur
His use of the word "helix" as a third helix was clearly and consistently with the context of:
. . . being invisible and occupying a displacement space called the Major Groove which is located in between the two helical strands.
What you did was lump the third helix together with the known 2 physical strands of DNA and accuse Rodin of being delusional.
DNA is not comprised of two helices but from three with the third helix being invisible
Selective splicing of novel DNA sequence combinations are able to be performed at will for medical treatment and the elimination of all diseases by utilizing these higher dimensional Flux Fields to control DNA cleavage and receptor sites.
Rodin did that himself and you edited the quote to try to make it look otherwise. Here is the full quote:
Originally posted by Mary Rose
reply to post by Arbitrageur
His use of the word "helix" as a third helix was clearly and consistently with the context of:
. . . being invisible and occupying a displacement space called the Major Groove which is located in between the two helical strands.
What you did was lump the third helix together with the known 2 physical strands of DNA
I still don't see any material difference, yes I said the third helix had never been seen and Rodin said it was invisible. How is that any different? It's not.
DNA is not comprised of two helices but from three with the third helix being invisible and occupying a displacement space called the Major Groove which is located in between the two helical strands.
Isn't he? Did he present any evidence for his invisible helix?
and accuse Rodin of being delusional.
Originally posted by Mary Rose
reply to post by buddhasystem
The third helix is clearly associated with magnetism, and energy healing.
Magnetism is different from physical strands.
Originally posted by buddhasystem
According to the Rodin's paper, it's associated with higher-dimensional bio-aetheric template, not some "magnetic field". Why are you putting words in Rodin's mouth? Why is it "clearly" associated? What's magnetism? If it's a magnetic field, what's causing it?
This Major Groove is not empty but instead is where all information for the genetic coding of life exists. . . . inside the Major Groove of DNA exists a higher dimensional Bioaetheric Template (Morphogenetic Field) definded by the mathematical number pattern 3, 9; 6; 6, 9, 3 revealing the existence of an All Coherent higher intelligence guiding evolution.
. . . Maxwell previously stated that there must needs to exist a Monopole to symmetrize all of his electrical equations. Rodin's math is a blueprint that gives us the ability to observe this missing Monopole in the form of an Inertia Aether Flux represented by the number 9 and its associated magnetic Field represented by the numbers 3 & 6 which are perfectly nested in unison within the Major Groove of DNA.
. . . phosphates are known to always have a negative electric charge and obviously with any negative electric charge there is always going to be an associated magnetic field.
Originally posted by Mary Rose
Originally posted by buddhasystem
According to the Rodin's paper, it's associated with higher-dimensional bio-aetheric template, not some "magnetic field". Why are you putting words in Rodin's mouth? Why is it "clearly" associated? What's magnetism? If it's a magnetic field, what's causing it?
Yes, you can substitute "magnetic field" for the word "magnetism," the word I used.
. . . phosphates are known to always have a negative electric charge and obviously with any negative electric charge there is always going to be an associated magnetic field.