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Regarding lung cancer, a methanolic extract of the leaves of Xanthium strumarium L. (Asteraceae) exhibited a strong inhibition of the proliferation of cultured human tumor cells, including A549 NSCLC cell line. The active constituents have been identified as 8-epi-xanthatin (Figure 1) and its epoxide, two xanthanolide sesquiterpene lactones. Their IC50 values have been calculated as 4.5 and 3.0 microM respectively, where the positive control cisplatin was 4.7 microM. (IC50 is the concentration of a compound
needed to reduce growth of a population of cells by 50 percent in vitro. At higher concentrations (64 and 58 mM, respectively) the two xantholides
showed a promising farnesyltransferase (FTase) inhibitory effect. 21 Farnesylation of certain oncoproteins (especially Ras proteins) is required for their oncogenic activity, and thus FTase inhibition could specifically stop Ras-mediated cellular proliferation. Synthetic FTase inhibitors have demonstrated activity against various human cancer cell lines, including NSCLC.22 An earlier study showed X. strumarium extracts are able to effec-
tively inhibit tubuline polymerization in mammalian tissues,23 which could be a plausible explanation of these findings.
The CKII inhibitory compound was purified from the fruit of Xanthium strumarium by organic solvent extraction and silica gel chromatography. The inhibitory compound was identified as 3,4-dihydroxybenzaldehyde by analysis with FT-IR, FAB-Mass, EI-Mass, (1)H-NMR and (13)C-NMR. 3,4-dihydroxybenzaldehyde inhibited the phosphotransferase activity of CKII with IC(50) of about 783 microM. Steady-state studies revealed that the inhibitor acts as a competitive inhibitor with respect to the substrate ATP. A value of 138.6 microM was obtained for the apparent K(i). Concentration of 300 microM 3,4-dihydroxybenzaldehyde caused 50% growth inhibition of human cancer cell U937. 3,4-dihydroxybenzaldehyde-induced cell death was characterised with the cleavage of poly(ADP-ribose) polymerase and procaspase-3. Furthermore, the inhibitor induced the fragmentation of DNA into multiples of 180 bp, indicating that it triggered apoptosis. This induction of apoptosis by 3,4-dihydroxybenzaldehyde was also confirmed by using flow cytometry analysis. Since CKII is involved in cell proliferation and oncogenesis, these results suggest that 3,4-dihydroxybenzaldehyde may function by inhibiting oncogenic disease, at least in part, through the inhibition of CKII activity.
In antioxidant activity assay such as DPPH radical and hydroxyl radical scavenging assay, Fe(2+) chelating assay, and intracellular ROS scavenging assay by DCF-DA, 3,4-dihydroxybenzaldehyde was found to scavenge DPPH radical, hydroxyl radical and intracellular ROS. Also it chelated Fe(2+). In in vitro oxidative DNA damage assay and the expression level of phospho-H2A.X, it inhibited oxidative DNA damage and its treatment decreased the expression level of phospho-H2A.X. And in oxidative cell death and apoptosis assay via MTT assay and Hoechst 33342 staining, respectively, the treatment of 3,4-dihydroxybenzaldehyde attenuated H(2)O(2)-induced cell death and apoptosis. These results suggest that the barley may exert the inhibitory effect on H(2)O(2)-induced tumor development by blocking H(2)O(2)-induced oxidative DNA damage, cell death and apoptosis.
The phenolic components isolated from the 80% MeOH extracts had markedly greater cancer cell toxicity than the extracts themselves. In particular, two out of seven compounds showed strong cytotoxicity towards several tumor cell lines without giving rise to significant cell toxicity toward normal cells. For example, the 50% lethal dose for 3,4-dihydroxybenzalacetone was 12.2μmol/L in PA-1 cells but was 272.8μmol/L in IMR90 cells. Fluorescence-activated cell sorting analysis further revealed these phenolic ingredients have high potentiality for apoptosis induction in PA-1 cells.
Originally posted by mnmcandiez
reply to post by rogerstigers
Diabetes is NOT cancer. You can make diabetes go away just by losing weight and eating healthy. My sister did this after she got a lap band. To compare diabetes and cancer is ridiculous.
Originally posted by Nivcharah
I recommend you read my previous post on this page, as the "Conquering ANY Disease" book has methods that have even reversed Diabetes TYPE I. It's even baffled medical physicians!
Originally posted by rogerstigers
Of course, I do not pay attention to anyone who is trying to sell a technique with a blender that they also happen to sell. Been hoodwinked too many times.
Originally posted by glitchinmymatrix
Acai berries? They aren't even a "superfood" much less a cancer cure.
Originally posted by glitchinmymatrix
I hope nobody here really goes on to cure anyone with some cinnamon and vanilla tea sweetened with honey while smoking a joint!
Originally posted by glitchinmymatrix
This is a tactic I'm familiar with that involves presenting so much information that people really can't practically investigate the claims, and even if they wanted to, it would take weeks.
I hope nobody here really goes on to cure anyone with some cinnamon and vanilla tea sweetened with honey while smoking a joint!