a reply to:
incoserv
These issues are most likely caused by a multitude of problems we did not predict.
Different people react to things being injected completely different than another person will. Our research was derived from animal models and
clinical trials. Animal models are homogeneous - this means you ask a specific question - find a genetic match like the hK18ACE2 B6 mouse - and then
challenge that question with a specific variable - CoV spike protein. Yeah it works great in them. We dose them with high viral titers, these mice are
bred to die with exposure from high affinity receptor and spike binding - vaccine stops that - move it to the next level.
Clinical trials follow once its cleared in non-human primates. Weird thing here is exclusion criteria - indirectly making the clinical trial itself
homogeneous to the question being asked because we exclude most of the population. Its why the efficacy rates were so high but its also something the
FDA (at least in the US) recommends.
Vaccine in the wild was mediocre, made infection worse in some, saved others, and people reacted to the injections. We still rushed it because there
was panic for a mild-moderate respiratory virus plus probably shareholders and $$$.
Viral vector vaccines are a stop gap measure because people will eventually attack the attenuated virus carrying an incorporated nucleic acid sequence
that produces your vaccine target antigen protein. The viral vector and now self amplifying RNA can still produce non-structural proteins (viral
replication proteins) that facilitate antigen production. People react to that - it can kill people - it can save people.
Lipid nanoparticle trafficking. Who would have thought. Lipid nanoparticles can traffic to specific tissues. We saw gonads and endothelial tissue in
the wild - not in our homogeneous mice or our clinical trials - even though it still trafficked once we went back and checked. LNP carries a slight
positive charge - they can carry negatively charged RNA or DNA - mRNA in the case of our vaccine.
Basically - you generate your RNA from synthetic or bacterial derived plasmids that you initially engineer through cloning to generate your antigen.
You cut the desired sequence from the plasmid - expand - package in an alcohol to facilitate the negative charge. You then pick a series of lipids -
derived from cell membranes - and you break them up via preset percentages and mix in a special cassette or machine. Because they both carry different
charge - you get encapsulation of the RNA inside the lipid. Extract the alcohol. Now, depending on the lipids you pick - something unexpected happens
when they are injected.
Trafficking to specific tissues. This happens because the LNP could have an affinity for transport proteins in our bodies and they can carry the LNP
and its payload to specific tissues. It goes in the wrong place - people get sick - people get complications - it can make infection worse. Goes to
the correct tissue - you usually save them. New vaccines will account for this but we are still years away from perfecting this. Anticancer
implications will be huge as will mucosal protection against respiratory viruses.
ACE2 receptor binding via spike protein or virus can indirectly activate pathways that stimulate inflammation because of receptor triggering of
downstream pathways inside the cell. Combined with interferon stimulated genes - potentially lots of inflammation.
Viral and vaccine spike and other proteins can move via endosomes and stimulate inflammatory pathways in tissue. These are similar to lipid
nanoparticles and are naturally released by cells to facilitate cell messaging - kind of like FedEx or UPS in cells.
Patients may have poor cytotoxic T-cell responses. These cells are responsible for viral clearance. Antibodies blunt infection - T-cells clear
infection along with some help from innate cells like natural killer cells and other antigen presenting cells. T-helper cells regulate all of this -
either good or bad. Vaccines and medications can influence these pathways through stimulating intrinsic defenses like interferon antiviral pathways.
Depending on how you do this - you can blunt infection and inflammation - or you can make it significantly worse.
LNP and mRNA are both very inflammatory and stimulate interferon very well. So much so that they are now considered adjuvants. Its why we use
psuedouridine instead of natural uridine in the mRNA vaccines, the cell will destroy it otherwise. Vulnerable patients can have a persistent infection
where the body reacts to low grade viral replication without clearance or it goes after protein targets from the host - meaning your cells
accidentally target a similar host protein or something that may have been near viral protein.
In these patients - these older patients - you have reactivation of virus - reactivation of latent virus, most likely herpes viruses like
Cytomegalovirus or Epstein barr virus. Secondary bacterial infection. Microbiota changes which virus and vaccine can impact through trafficking and
inflammation. There is the Long COVID issue - this could be related to vaccines, CoV-2, or a combination of both along with comorbidities and
inflammation in the patient. Then you have to look at the epigenetic impact of everything in these patients that alters gene expression. That is DNA
stays the same - it is just packaged differently in the cell and expressed differently because of the environment the host is exposed to. So you now
have abnormal protein expression even though nothing changed except for some methylation or other change in how the DNA can be accessed by DNA binding
proteins when they are triggered by messengers to generate RNA for proteins. It basically speeds up how quickly you get cancer that bypasses our
immune defenses - everyone has cancer - its just when it escapes that you have problems.
I was on here alot during the pandemic - lost access and now hope to participate again. Thinking of a few threads to easily explain these pathways -
especially since I denied most of them initially and now we see them in the lab as we fine tune our research in models. Hopefully some of this makes
sense - if not ask away. Please. There is no black and white with this virus or these vaccines.