Help me understand the mRNA technology

I am trying to understand how the injections work. I have no background in biology, and the small bit I took is decades away now.

My current understanding is that the injections contained billions of small lipid particles carrying mRNA. The lipid covering allows the mRNA to enter a cell, as normally free floating mRNA would be rejected at the cell wall. The lipid particles are able to cross the blood brain barrier. Once inside a cell, the mRNA will instruct the cell to manufacture a spike protein. The spike protein is found on the surface of the virus and allows the virus into the cell by docking with the ACE2 receptor. Spike protein is foreign to the body and any cell manufacturing soike protein will alert the bodies immune system and be marked for destruction.

Please elaborate or correct me if I am wrong.

The following ideas are things I've heard about, but not sure if this is true.

The synthetic mRNA that is found in the injection does not break down as naturally occurring mRNA would in a matter of hours, but has been found to continue manufacturing spike protein up to 120 days. Once the cell it has taught to manufacture spike is destroyed, the macrophages do not know how to recycle it? I'm not sure how it would again be able to enter another cell without the lipid.

Injected lipid mRNA will circulate through the body and can accumulate in any organ. Heart muscle that is taught to express spike will be targeted by the immune system, and when killed will turn into permanent scar tissue on the heart. This process will also cause inflamation of the heart.

The spike protein manufacturing instructions are based on the alpha variant of the virus. The current virus spike has mutated, becoming less pathogenic, but also making all current injections irrelevant.

If you can offer me more information, or correction in my understanding, it would be appreciated.

a reply to: Zenchuck

This should be good. I don't think you gave them much opening for sniping on irrelevant side info. My guess is they try to paint you as a loon by attributing every conspiracy theory about COVID as your words.

I look forward to multiple complicated posts that don't answer your questions while explaining other things you didn't ask in a condescending manner.

That is correct.

a reply to: Ksihkehe

Thanks. I am not looking for an arguement, as I have no real understanding of the topic. Just looking for clarification.

For example. If the lipid can cross the blood brain barrier and is carrying instructions for cell destruction. Would this then cause brain damage?
Likewise, the lipid has been found to accumulate in ovaries and testes. Would the resulting damages possibly lead to infertility?

This info was a interesting read ....covers info on how it works and or should work.


The Problem With mRNA Vaccines - Brightwork Research & Analysis - LiNk

When it was announced that untested technology called mRNA would be used to create vaccines for covid, the response was extremely positive. However, there are many problems with mRNA vaccines.

a reply to: Zenchuck

I am waiting for our resident medical experts, ARGGH and ChR0NO and Middle to come in and explain to this poor fellow.

a reply to: Zenchuck

It is also theorized by some researchers that the vaccine highjacks the immune system to make C-19 its priority, making the it ignore its normal function of protecting against normal job of fighting a host of different viruses. The old 'if you don't use it you'll loose it' scenario.

My understanding is the lipid particles can accumulate in and damage several different organs permanently. Outcome depends on the severity of the damage.

Of course, this is being debunked by all official sources, so it boils down to a trust issue for most people.

Do you believe the government has your best interests at heart? Do you believe the news tells the whole truth and nothing but the truth? Those members who do will be along shortly to tell us what gullible idiots we are.

originally posted by: Zenchuck
My current understanding is that the injections contained billions of small lipid particles carrying mRNA. The lipid covering allows the mRNA to enter a cell, as normally free floating mRNA would be rejected at the cell wall. The lipid particles are able to cross the blood brain barrier. Once inside a cell, the mRNA will instruct the cell to manufacture a spike protein. The spike protein is found on the surface of the virus and allows the virus into the cell by docking with the ACE2 receptor. Spike protein is foreign to the body and any cell manufacturing soike protein will alert the bodies immune system and be marked for destruction.

I think you meant cell wall instead of blood brain barrier.

The synthetic mRNA that is found in the injection does not break down as naturally occurring mRNA would in a matter of hours, but has been found to continue manufacturing spike protein up to 120 days. Once the cell it has taught to manufacture spike is destroyed, the macrophages do not know how to recycle it? I'm not sure how it would again be able to enter another cell without the lipid.

Pretty sure RNA is RNA regardless of how it is put together and it is heat that degrades it.

If there are any cases of people producing spike proteins 120 days after a jab, I'd say they probably were infected by the virus shortly before those tests. Like you said, without the lipid it isn't going to enter any other cells and at body temp it will degrade.

Injected lipid mRNA will circulate through the body and can accumulate in any organ. Heart muscle that is taught to express spike will be targeted by the immune system, and when killed will turn into permanent scar tissue on the heart. This process will also cause inflamation of the heart.

The shots are intramuscular and, although possible, I find it hard to believe that a significant amount, if any, would make it that far before entering another cell.

The spike protein manufacturing instructions are based on the alpha variant of the virus. The current virus spike has mutated, becoming less pathogenic, but also making all current injections irrelevant.

If we are honest, for the majority of the population the vaccinations were irrelevant even with the alpha variant.

originally posted by: nugget1
My understanding is the lipid particles can accumulate in and damage several different organs permanently. Outcome depends on the severity of the damage.

Lipid is just another word for fat. How much fat do you think there is in a .5ml dose of the vax?

There is probably more than 1000x the amount of lipids in a pat of butter.

originally posted by: daskakik

originally posted by: Zenchuck
My current understanding is that the injections contained billions of small lipid particles carrying mRNA. The lipid covering allows the mRNA to enter a cell, as normally free floating mRNA would be rejected at the cell wall. The lipid particles are able to cross the blood brain barrier. Once inside a cell, the mRNA will instruct the cell to manufacture a spike protein. The spike protein is found on the surface of the virus and allows the virus into the cell by docking with the ACE2 receptor. Spike protein is foreign to the body and any cell manufacturing soike protein will alert the bodies immune system and be marked for destruction.

I think you meant cell wall instead of blood brain barrier.

The synthetic mRNA that is found in the injection does not break down as naturally occurring mRNA would in a matter of hours, but has been found to continue manufacturing spike protein up to 120 days. Once the cell it has taught to manufacture spike is destroyed, the macrophages do not know how to recycle it? I'm not sure how it would again be able to enter another cell without the lipid.

Pretty sure RNA is RNA regardless of how it is put together and it is heat that degrades it.

If there are any cases of people producing spike proteins 120 days after a jab, I'd say they probably were infected by the virus shortly before those tests. Like you said, without the lipid it isn't going to enter any other cells and at body temp it will degrade.

Injected lipid mRNA will circulate through the body and can accumulate in any organ. Heart muscle that is taught to express spike will be targeted by the immune system, and when killed will turn into permanent scar tissue on the heart. This process will also cause inflamation of the heart.

The shots are intramuscular and, although possible, I find it hard to believe that a significant amount, if any, would make it that far before entering another cell.

The spike protein manufacturing instructions are based on the alpha variant of the virus. The current virus spike has mutated, becoming less pathogenic, but also making all current injections irrelevant.

If we are honest, for the majority of the population the vaccinations were irrelevant even with the alpha variant.

This sort of covers it

a reply to: Zenchuck

My current understanding is that the injections contained billions of small lipid particles carrying mRNA.

correct

The lipid covering allows the mRNA to enter a cell, as normally free floating mRNA would be rejected at the cell wall.

incorrect the lipids are to slow down how quickly the RNA breaks down unprotected it would be a couple of hours at most.

The lipid particles are able to cross the blood brain barrier.

incorrect they are too large I think you mean the spike proteins that are produced.

The spike protein is found on the surface of the virus and allows the virus into the cell by docking with the ACE2 receptor. Spike protein is foreign to the body and any cell manufacturing soike protein will alert the bodies immune system and be marked for destruction.

correct

The synthetic mRNA that is found in the injection does not break down as naturally occurring mRNA would in a matter of hours, but has been found to continue manufacturing spike protein up to 120 days.

incorrect again you are mistaking the spike protein for mRNA. The mRNA from the injection would be gone in about 72 hrs as the lipids break down and simply get torn apart.

Injected lipid mRNA will circulate through the body and can accumulate in any organ. Heart muscle that is taught to express spike will be targeted by the immune system

Possible however that requires the shot to be given incorrectly into the bloodstream. It is an intramuscular vaccine (many vaccines are that's why the injection site becomes sore) Vaccines mostly remain near the site of injection (the arm muscle) and local lymph nodes.

The spike protein manufacturing instructions are based on the alpha variant of the virus. The current virus spike has mutated, becoming less pathogenic, but also making all current injections irrelevant.xt

This one is tough to answer yes the virus mutates and yes that could lessen the effects of the vaccine (it does this by overwhelming our immune systems producing more than our immune system can handle)however it doesn't render it completely useless because one thing all the variants have in common is the spike protein. So your body will still recognize them as a foreign body. It just may not be able to produce enough antibodies to kill the virus and you could become very sick.

a reply to: daskakik

Really appreciate the response.

My understanding was the original lipid technology was specifically created to pass through the BBB, while it also passes through the cell wall. Original application was for neurological treatment.

The solution creating the synthetic RNA did not contain Uridine, but pseudouridine, which makes the RNA more stable. Naturally occurring pseudouridine is carefully regulated by the cell. Could this have an effect on the longevity of the RNA?

a reply to: Zenchuck

My Laymen Understanding of it is Artifically Created Prions inserted in these Civid-19 " Pseudo Vaccines " Somehow Alter a Humans DNA in Negative Ways , thus Effecting the Body's Natural Immune System which Causes the Body to Deteriorate Due to it's Reduced Effectiveness of Fighting Off Infections . An Induced Poison that Also Rots the Mind and Steals Ones Soul .....

a reply to: dragonridr

Thanks for the response.

Would you say the lipids protect the RNA and allow them entry to the cell? My understanding is that without this technology, the RNA would not enter a cell.

The technology is also described as 10 nanometers. The original tech was designed to cross the BBB. How is this application different? Have they designed these to be larger?

originally posted by: Zenchuck
My understanding was the original lipid technology was specifically created to pass through the BBB, while it also passes through the cell wall. Original application was for neurological treatment.

I think the size of what is being encapsulated would be the most important factor. Molecules vs rna is a big difference.

According to this the average size of the encapsulated mrna particles in this study was 100 nm.

We used the well-established lipid nanoparticles (LNPs) to package mRNAs. The mRNA encapsulation efficiency of all three LNP vaccine candidates was greater than 98%, with an average size of 100 nm in diameter (Supplementary information, Fig. S1b, c).

The solution creating the synthetic RNA did not contain Uridine, but pseudouridine, which makes the RNA more stable. Naturally occurring pseudouridine is carefully regulated by the cell. Could this have an effect on the longevity of the RNA?

I had not heard of that but the question is how long?

If it doubles the time it takes to degrade, that would be really something, but it would still be a question of hours.

I can't seem to find anything on the amount of time though.

a reply to: daskakik

Does size matter when crossing the BBB? Or what types of particles are accepted vs rejected - it looks like it's a lipid only club . I'll need to read more about this. My current understanding is that lipid nanoparticles were developed as a drug delivery system to the brain. I'm assuming this new use would have specifically excluded this feature, but then I run into the second assumption, that any contents of the shot will remain local to the shoulder in which it was injected. Could it be that the arguement supporting the inability to cross the BBB comes from the assumption that it would never have the opportunity?

On the second assumption, that substitution of a component would not change the downstream ability for that component to be disassembled, when one of the key features recognized in the substitution was the stability that it offered. There should be clear answers to these questions.

Your and other thoughtful responses are why I keep coming back to this site.

a reply to: daskakik

If IM shots aren't properly aspirated upon administration, as many folks have described when receiving their doses, then the syringe might've hit a blood vessel. Then instead of the dose sitting in depot in the muscle belly, it could get into the blood stream and all bets are off on where it can end up. Does this happen often? Probably not, but out of the "billions" of doses of this that were given, and the more than a few anecdotes of improper IM injection, without question the odds are this happened more than a few times, and this accounted for some of the insane stories of reactions.

How often did this happen with the flu shot? Hard to say, but then again, no flu vaccine on Earth was ever pimped this heard. When you go hog wild, mandating vaccines for a significant % of the planet's population that, for healthy individuals under the age of 65, the virus is 97%+ survivable, you've just shifted mortality shell game from COVID to other factors, improper administration like failing to aspirate, exposing people with allergic reactions to the non-mRNA ingredients of vaccines (who never had the flu vaccine shoved down their throats before), QC failures in the manufacturing process (graphene anyone?).

Even if, IF the vaccine is "100% safe and effective", and this narrative of completely safe and effective was written in permanent ink waaaay before sufficient data has been collected, the unnecessary over-medication of a manufactured vaccine, for the reasons above, killed people that might've have otherwise survived COVID.

I am thinking someday, enterprising data scientists and lawyers will do some studies to quantify how over-medicated this program was, model figures for how many folks were killed for purely administration/QC failures on the mfg of the vaccine, having nothing to even do with the mRNA, and they will (like good lawyers do) find chinks in Pfizer's iron clad "lawsuit protection" on this vaccine, and siphon off vast sums of money from the outrageous figures Pfizer slurped out of governments by colluding with "health professionals" to ram-rod this down the population's throats, whether needed or not.

Not that it will make anyone from Pfizer or the CDC go to jail, just put a small dent in their blood money profiteering.

a reply to: Zenchuck
According to this there does seem to be a physical size limit.
Possible strategies to cross the blood–brain barrier

Transport at the BBB
Generally, only lipid soluble (lipophilic) molecules with a low molecular weight (under 400–600 Da) and of positive charge can cross the BBB.

So it would seem, besides being lipid soluble, there is also a size limit.

So while you are right that lipid nanoparticles were developed as a drug delivery system to the brain, what where the sizes of these particles?