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In Vivo Infection of Human ACE2 (hACE2) Expressing Mice with SARSr-CoV S Protein variants Using the reverse genetic methods we previously developed, infectious clones with the WIV1 backbone and the spike protein of SHC014, WIV16 and Rs4231, respectively, were constructed and recombinant viruses were successfully rescued. In Year 4, we performed preliminary in vivo infection of SARSr-CoVs on transgenic mice that express hACE2. Mice were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. Pathogenesis of the 4 SARSr-CoVs was then determined in a 2-week course. Mice challenged with rW IV1-SHC014S have experienced about 20% body weight loss by the 6th day post infection, while rWIV1 and rWIV-4231 S produced less body weight loss. In the mice infected with rWIV1-WIV16S, no body weight loss was observed (Fig. 35a). 2 and 4 days post infection, the viral load in lung tissues of mice challenged with rWIV1-SHC014S, rWIV1-WIV16S and rWIV1-Rs4231 S reached more than 106genome copies/g and were significantly higher than that in rWIV1-infected mice (Fig. 35b). These results demonstrate varying pathogenicity of SARSr-CoVs with different spike proteins in humanized mice.
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Sharon Lerner, Mara Hvistendahl
September 6 2021, 7:06 p.m.
Newly released documents provide details of U.S.-funded research on several types of coronaviruses at the Wuhan Institute of Virology in China. The Intercept has obtained more than 900 pages of documents detailing the work of EcoHealth Alliance, a U.S.-based health organization that used federal money to fund bat coronavirus research at the Chinese laboratory. The trove of documents includes two previously unpublished grant proposals that were funded by the National Institute of Allergy and Infectious Diseases, as well as project updates relating to EcoHealth Alliance’s research, which has been scrutinized amid increased interest in the origins of the pandemic.
The documents were released in connection with ongoing Freedom of Information Act litigation by The Intercept against the National Institutes of Health. The Intercept is making the full documents available to the public.
“This is a road map to the high-risk research that could have led to the current pandemic,” said Gary Ruskin, executive director of U.S. Right To Know, a group that has been investigating the origins of Covid-19.
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The bat coronavirus grant provided EcoHealth Alliance with a total of $3.1 million, including $599,000 that the Wuhan Institute of Virology used in part to identify and alter bat coronaviruses likely to infect humans. Even before the pandemic, many scientists were concerned about the potential dangers associated with such experiments. The grant proposal acknowledges some of those dangers: “Fieldwork involves the highest risk of exposure to SARS or other CoVs, while working in caves with high bat density overhead and the potential for fecal dust to be inhaled.”
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Chinese scientists believe the deadly coronavirus may have started life in a research facility just 300 yards from the Wuhan fish market.
A new bombshell paper from the Beijing-sponsored South China University of Technology says that the Wuhan Center for Disease Control (WHCDC) could have spawned the contagion in Hubei province.
'The possible origins of 2019-nCoV coronavirus,' penned by scholars Botao Xiao and Lei Xiao claims the WHCDC kept disease-ridden animals in laboratories, including 605 bats.
It also mentions that bats - which are linked to coronavirus - once attacked a researcher and 'blood of bat was on his skin.'
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originally posted by: ElectricUniverse
This research states quite clearly that they wanted to weaponize and make coronaviruses more lethal...
originally posted by: EvilAxis
Where does it say that?
Richard H. Ebright is an American molecular biologist. He is the Board of Governors Professor of Chemistry and Chemical Biology at Rutgers University and Laboratory Director at the Waksman Institute of Microbiology.
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Back in October 2014, the US government had placed a federal moratorium on gain-of-function (GOF) research – altering natural pathogens to make them more deadly and infectious – as a result of rising fears about a possible pandemic caused by an accidental or deliberate release of these genetically engineered monster germs.
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As such in October 2014, because of public health concerns, the US government banned all federal funding on efforts to weaponize three viruses – influenza, Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome (SARS).
In the face of a moratorium in the US, Dr Anthony Fauci – the director of the National Institute of Allergy and Infectious Diseases (NIAID) and currently the leading doctor in the US Coronavirus Task Force – outsourced in 2015 the GOF research to China’s Wuhan lab and licensed the lab to continue receiving US government funding.
The Wuhan lab is now at the center of scrutiny for possibly releasing the SARS-CoV-2 coronavirus and causing the global Covid-19 pandemic.
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originally posted by: EvilAxis
a reply to: ElectricUniverse
I'm not disputing what they did or what you or other virologists might surmise from it.
You said, "This research states quite clearly that they wanted to weaponize and make coronaviruses more lethal..."
It doesn't.
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2 and 4 days post infection, the viral load in lung tissues of mice challenged with rWIV1-SHC014S, rWIV1-WIV16S and rWIV1-Rs4231 S reached more than 106 genome copies/g and were significantly higher than that in rWIV1-infected mice (Fig. 35b). These results demonstrate varying pathogenicity of SARSr-CoVs with different spike proteins in humanized mice.
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originally posted by: ElectricUniverse
...again it does prove intent.
originally posted by: EvilAxis
It wasn't proving intent that I challenged you about. That's a different and arguable matter. They did not, as you claimed, "state quite clearly that they wanted to weaponize" the virus.
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Released five years before the start of the COVID-19 pandemic, it describes SARS coronaviruses as a “new era of genetic weapons” that can be “artificially manipulated into an emerging human disease virus, then weaponised and unleashed in a way never seen before”.
Peter Jennings, the executive director of the Australian Strategic Policy Institute (ASPI), told news.com.au that the document is as close to a “smoking gun” as we’ve got.
“I think this is significant because it clearly shows that Chinese scientists were thinking about military application for different strains of the coronavirus and thinking about how it could be deployed,” said Mr Jennings.
“It begins to firm up the possibility that what we have here is the accidental release of a pathogen for military use,” added Mr Jennings.
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originally posted by: EvilAxis
a reply to: ElectricUniverse
I know what they did, and believe the danger outweighed the possible benefit.
The rationale given for the research was to predict possible mutational outcomes of a wild virus by performing them in a lab.
They did not state "quite clearly that they wanted to weaponize and make coronaviruses more lethal..." You surmise it.
Wuhan lab researcher linked to military scientists, NBC News finds
June 29, 2021
In January, a Trump administration fact sheet accused China of “secret military activity” at a lab in Wuhan. Dr. Shi Zhengli, a leading researcher at the Wuhan Institute of Virology, says it is a civilian institution, but NBC News has found evidence of her connections with military scientists.
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originally posted by: ElectricUniverse
a reply to: Ghostsdogood
He/she is probably going to spew Chinese military propaganda/far left msn propaganda. There is no distinction between the two now imo, same with Hollywood and sports agencies whom are kowtowing to the ChInese dictatorship...
Of course, Chinese officials, and at least some scientists, have claimed the Wuhan labs are civilian... Which is BS since China's government and military arm has control over everything in China.
The lying msn/mainstream media tried to change course, but not enough, when they published this.
Wuhan lab researcher linked to military scientists, NBC News finds
June 29, 2021
In January, a Trump administration fact sheet accused China of “secret military activity” at a lab in Wuhan. Dr. Shi Zhengli, a leading researcher at the Wuhan Institute of Virology, says it is a civilian institution, but NBC News has found evidence of her connections with military scientists.
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Wuhan lab researcher linked to military scientists, NBC News finds