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originally posted by: chr0naut
The majority of geneticists and virologists have said that the creation of SARS-CoV-2 in a lab is unlikely, but no-one can be entirely sure. Despite this, there are people who for sensationalist or political reasons promote the idea.
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Sharon Lerner, Mara Hvistendahl
September 6 2021, 7:06 p.m.
Newly released documents provide details of U.S.-funded research on several types of coronaviruses at the Wuhan Institute of Virology in China. The Intercept has obtained more than 900 pages of documents detailing the work of EcoHealth Alliance, a U.S.-based health organization that used federal money to fund bat coronavirus research at the Chinese laboratory. The trove of documents includes two previously unpublished grant proposals that were funded by the National Institute of Allergy and Infectious Diseases, as well as project updates relating to EcoHealth Alliance’s research, which has been scrutinized amid increased interest in the origins of the pandemic.
The documents were released in connection with ongoing Freedom of Information Act litigation by The Intercept against the National Institutes of Health. The Intercept is making the full documents available to the public.
“This is a road map to the high-risk research that could have led to the current pandemic,” said Gary Ruskin, executive director of U.S. Right To Know, a group that has been investigating the origins of Covid-19.
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The bat coronavirus grant provided EcoHealth Alliance with a total of $3.1 million, including $599,000 that the Wuhan Institute of Virology used in part to identify and alter bat coronaviruses likely to infect humans. Even before the pandemic, many scientists were concerned about the potential dangers associated with such experiments. The grant proposal acknowledges some of those dangers: “Fieldwork involves the highest risk of exposure to SARS or other CoVs, while working in caves with high bat density overhead and the potential for fecal dust to be inhaled.”
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Chinese scientists believe the deadly coronavirus may have started life in a research facility just 300 yards from the Wuhan fish market.
A new bombshell paper from the Beijing-sponsored South China University of Technology says that the Wuhan Center for Disease Control (WHCDC) could have spawned the contagion in Hubei province.
'The possible origins of 2019-nCoV coronavirus,' penned by scholars Botao Xiao and Lei Xiao claims the WHCDC kept disease-ridden animals in laboratories, including 605 bats.
It also mentions that bats - which are linked to coronavirus - once attacked a researcher and 'blood of bat was on his skin.'
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originally posted by: chr0naut
The evidence you provided was evidence of funding, not of gain of function.
The articles you linked to mentioned of "gain of function", and of "altering" viruses, but, as near as I can tell, their source information made no actual mention of it. They seem to have merely assumed that because there was funding, that it must have been for GOF research.
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TYPES OF GAIN-OF-FUNCTION (GOF) RESEARCH
Subbarao explained that routine virological methods involve experiments that aim to produce a gain of a desired function, such as higher yields for vaccine strains, but often also lead to loss of function, such as loss of the ability for a virus to replicate well, as a consequence. In other words, any selection process involving an alteration of genotypes and their resulting phenotypes is considered a type of Gain-of-Function (GoF) research, even if the U.S. policy is intended to apply to only a small subset of such work.
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originally posted by: ElectricUniverse
originally posted by: chr0naut
The evidence you provided was evidence of funding, not of gain of function.
The articles you linked to mentioned of "gain of function", and of "altering" viruses, but, as near as I can tell, their source information made no actual mention of it. They seem to have merely assumed that because there was funding, that it must have been for GOF research.
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Why do you have to make claims about GOF research when it is obvious you know nothing about it?
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TYPES OF GAIN-OF-FUNCTION (GOF) RESEARCH
Subbarao explained that routine virological methods involve experiments that aim to produce a gain of a desired function, such as higher yields for vaccine strains, but often also lead to loss of function, such as loss of the ability for a virus to replicate well, as a consequence. In other words, any selection process involving an alteration of genotypes and their resulting phenotypes is considered a type of Gain-of-Function (GoF) research, even if the U.S. policy is intended to apply to only a small subset of such work.
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Gain-of-Function Research: Background and Alternatives
When research is done to make coronaviruses more lethal and infectious to humans that is Gain of Function Research...
Which is what the Obama administration, Fauci, and company wanted when they outsourced such dangerous experiments to China... The world's most dictatorial system which has murdered millions of their own people that the communist dictatorship didn't want...
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In Vivo Infection of Human ACE2 (hACE2) Expressing Mice with SARSr-CoV S Protein variants Using the reverse genetic methods we previously developed, infectious clones with the WIV1 backbone and the spike protein of SHC014, WIV16 and Rs4231, respectively, were constructed and recombinant viruses were successfully rescued. In Year 4, we performed preliminary in vivo infection of SARSr-CoVs on transgenic mice that express hACE2. Mice were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. Pathogenesis of the 4 SARSr-CoVs was then determined in a 2-week course. Mice challenged with rW IV1-SHC014S have experienced about 20% body weight loss by the 6th day post infection, while rWIV1 and rWIV-4231 S produced less body weight loss. In the mice infected with rWIV1-WIV16S, no body weight loss was observed (Fig. 35a). 2 and 4 days post infection, the viral load in lung tissues of mice challenged with rWIV1-SHC014S, rWIV1-WIV16S and rWIV1-Rs4231 S reached more than 106genome copies/g and were significantly higher than that in rWIV1-infected mice (Fig. 35b). These results demonstrate varying pathogenicity of SARSr-CoVs with different spike proteins in humanized mice.
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originally posted by: ElectricUniverse
a reply to: chr0naut
In page 298 you can read this.
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In Vivo Infection of Human ACE2 (hACE2) Expressing Mice with SARSr-CoV S Protein variants Using the reverse genetic methods we previously developed, infectious clones with the WIV1 backbone and the spike protein of SHC014, WIV16 and Rs4231, respectively, were constructed and recombinant viruses were successfully rescued. In Year 4, we performed preliminary in vivo infection of SARSr-CoVs on transgenic mice that express hACE2. Mice were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. were infected with 105 pfu of full-length recombinant virus of WIV1 (rWIV1) and the three chimeric viruses with different spikes. Pathogenesis of the 4 SARSr-CoVs was then determined in a 2-week course. Mice challenged with rW IV1-SHC014S have experienced about 20% body weight loss by the 6th day post infection, while rWIV1 and rWIV-4231 S produced less body weight loss. In the mice infected with rWIV1-WIV16S, no body weight loss was observed (Fig. 35a). 2 and 4 days post infection, the viral load in lung tissues of mice challenged with rWIV1-SHC014S, rWIV1-WIV16S and rWIV1-Rs4231 S reached more than 106genome copies/g and were significantly higher than that in rWIV1-infected mice (Fig. 35b). These results demonstrate varying pathogenicity of SARSr-CoVs with different spike proteins in humanized mice.
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Understanding-Risk-Bat-Coronavirus-Eme rgence-Grant-Notice
You do remember what "chimeric viruses" are right?
They isolated different traits, spike protein from 3 different strains of chimeric coronaviruses. They genetically modified them(adding the different traits from different strains of chimeric coronaviruses) to construct a new recombinant virus. (which is also a chimeric virus)
SHC014-CoV is a SARS-like coronavirus which infects horseshoe bats.
en.wikipedia.org...
WIV16 is another strain of SARS like coronavirus.
www.viprbrc.org...
Rs4231 is another strain of SARS-related coronavirus.
Then they infected mice with ACE2 human receptors with the reconbinant virus. This was Gain of Function Research despite Fauci and company lying about it. The goal was to make a coronavirus that would be more lethal and infectious to humans.
BTW, do note that they state these three strains are chimeric. Hence the three strains are not natural strains, and neither is the recombinant virus they produced from the three chimeric strains of coronaviruses.
originally posted by: chr0naut
What they are doing here is taking the spike protein sequence and add it to a benign virus. One which will not cause disease. This is very similar to the way the AstraZaneca vaccine delivers the mRNA sequence to the ribosome, but in this case the adapted virus includes the protein itself, and not just the mRNA that codes for the protein. The resultant virus is chimeric (which the CDC is now putting a ban on) but it isn't the potentially pathogenic virus that is being modified, but is a known benign virus (usually an adenovirus).
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Richard H. Ebright is an American molecular biologist. He is the Board of Governors Professor of Chemistry and Chemical Biology at Rutgers University and Laboratory Director at the Waksman Institute of Microbiology.
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originally posted by: ElectricUniverse
On November 17th 2021 the CDC made the following announcement about SARS/CoV-2.
Today, the Centers for Disease Control and Prevention (CDC)’s Division of Select Agents and Toxins published an Interim Final Rule adding SARS-CoV/SARS-CoV-2 chimeric viruses resulting from any deliberate manipulation of SARS-CoV-2 to incorporate nucleic acids coding for SARS-CoV virulence factors to the list of HHS select agents and toxins. In addition, the work to create this chimeric virus is a ‘restricted experiment’ and requires prior approval from CDC before performing the experiment.
The regulation, available at www.federalregister.gov... icon, was published in the Federal Register and CDC will be accepting public comments on the addition of the agent for the next 60 days.
HHS/CDC believes that immediate regulatory oversight of these experiments and the resulting chimeric viruses is essential to protect the public from the potential consequences of a release of these viruses.
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Import Permit Program (IPP) SARS/CoV-2
Here is the definition of Chimeric:
chi·mer·ic (kī-mĕr′ĭk, -mîr′-)
adj.
1. Relating to or being an organism, part, or molecule that is a chimera: chimeric mice; chimeric proteins.
2. Relating to a monoclonal antibody produced from the cells of a nonhuman organism, usually a mouse, in which a portion of the antibody has been replaced with a human sequence of amino acids. This is done in the laboratory by replacing part of the DNA sequence in the nonhuman cells with a sequence of human DNA.
chimeric
Remember that when president Trump stated we had evidence this virus was created in a Wuhan lab in China the mainstream media and even democrat leaders, the WHO, etc all claimed this statement by President Trump was based on "racism" and not on fact.
Well, on November 17th 2021 the CDC added the SARS/CoV-2 viruses as a chimeric deliberate manipulation, and any further manipulation of the virus has now to get the go ahead of the CDC.
Since almost the start of this plandemic we started getting information that proved the bio-weapons program to make SARS/Coronaviruses more lethal and infectious to humans was outsourced on purpose under the Obama administration and with the help of Fauci.
There is no other way the outsourcing of this virus to a communist dictatorship that has murdered millions of their own people on purpose had any other intention by Fauci and the Obama/Biden administration than to murder as many people as possible through such viruses. The claim by the Obama administration and Fauci that outsourcing these bio-weapons program to the U.S. and world's number 1 enemy, China, was to avoid any accidental release of the virus was an obvious lie because China has a history of more accidental release of viruses from labs than the U.S. has had.
Since the virus escaped apparently before they were able to weaponize it more then the goal was moved to make the vaccines as the delivery method to continue such bio-weapons program. Anyone who dared/dares release info on this bio-weapons program by China, the Obama administration and the Fauci's of the world has found themselves to be shunned from "the scientific community", has been denied funding, etc, and now the CDC is once again making it impossible for independent scientists to investigate this virus without the consent from the CDC itself which has been lying and continues to lie day in and day out about this plandemic.
Now no scientist/scientific group can investigate how to fight this virus or attempt to make any vaccines/mutate the virus unless they have permission from the CDC. Why?
originally posted by: sarahvital
oh thank god!
i thought my hamsters were up to no good again!
originally posted by: ElectricUniverse
originally posted by: sarahvital
oh thank god!
i thought my hamsters were up to no good again!
Care to provide anything of substance and intelligent to the topic? Or is your entire premise to just dismiss the evidence you apparently don't want to accept?
originally posted by: chr0naut
The resultant virus was chimeric, but they did not alter the source strains. They took the spike proteins from them and applied them to a benign virus which would not be a viable replicable reinfector.
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2 and 4 days post infection, the viral load in lung tissues of mice challenged with rWIV1-SHC014S, rWIV1-WIV16S and rWIV1-Rs4231 S reached more than 106 genome copies/g and were significantly higher than that in rWIV1-infected mice (Fig. 35b). These results demonstrate varying pathogenicity of SARSr-CoVs with different spike proteins in humanized mice.
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originally posted by: Antisocialist
a reply to: ElectricUniverse
This plandemic is just the first act, an experiment, in global population control.
Act 2, or the next plandemic will be a REAL killer. Expect it!
originally posted by: ElectricUniverse
originally posted by: sarahvital
oh thank god!
i thought my hamsters were up to no good again!
Care to provide anything of substance and intelligent to the topic? Or is your entire premise to just dismiss the evidence you apparently don't want to accept?
originally posted by: chr0naut
a reply to: ElectricUniverse
This is not saying that SARS-CoV-2 is a chimeric human made virus.
It is a ruling that SARS-CoV-2 is not to be used in future human made viruses. It is a ban on future experimentation that may use the virus as a basis.
originally posted by: zatara
originally posted by: Antisocialist
a reply to: ElectricUniverse
This plandemic is just the first act, an experiment, in global population control.
Act 2, or the next plandemic will be a REAL killer. Expect it!
No matter how much I would like to deny I am afraid that you are right. The entire introduction of the virus to the world went like a procedure in progress... The media played a huge role in the scare tactics which made the population scream for a swift solution... A classic case of Hegelian Dialectic, problem-reaction-solution. It went all to organised and prepared... Its like an other 911, a global random striking enemy you can not see and potentially deadly.
There was also a part which was designed to condition the people, to make them familiar with the treath and measures to fight the pandemic. Yes...something real ugly is coming our way..