Gene editing with great potential, or Pandoras Box?

As we leap forward in all fields of research, I'd like to address a rather new technology called CRISPR/CAS9. 
It is a revolutionary gen editing mechanism. 

It all started with the discovery of CRISPR.
. Wiki

 (which is an acronym for clustered regularly interspaced short palindromic repeats)

These are short gen sequences that are identical and repeat in constant intervals. 

What they found out is, part of a cell immune system uses these sequences to create immunity. What happens when a pathogen enters the cell, the cas9 protein scans it then searches for a fitting sequence. It cuts part of the pathogen genetic code and adds it to the cell genome.

.wiki CAS9

This added genetic information from the pathogen makes it that any pathogen with that same genetic marker can not attach to the cell and thus making it immune. 

The role of the CAS9 protein was only discovered recently, but led to a new way of editing genomes. They have come far since then and are now able to repair damaged genes, activate or deactivate certain genes, introduce new gene sequences.

Heck China did Their own CRISPR babies

Back in November 2018, when He first announced the existence of the gene-edited twins, he also let slip that another woman was pregnant with an additional edited embryo.

. article

There is even an enterprise that sells CRISPR/CAS9 DIY home kits.

At The ODIN, we believe the future is going to be dominated by genetic engineering and consumer genetic design will be a big part of that. We are making that happen by creating kits and tools that allow anyone to make unique and usable organisms at home or in a lab or anywhere.

Odin DIY kit

This is a very simple breakdown and only what I understand of it, correct me if wrong.



There is this opinion piece of wannabe documentary on the topic.



As far as I understand the COVID19 "Vaccines" are working with the CRISPR/CAS9 technologie. While the Shot is supposed to only introduce a modified CAS9 protein, that has the COVID19 sequence....

What really gave me pause is the very common use for a virus, in the CRISPR/CAS9 research, to introduce new gene sequences.
You don't have to be a genius to see the potential for conspiracies we have here.

A possibly lab made virus, a new gene editing technology that uses Viruses to introduce new Gene sequences.
And it all comes hegelianly together with an untested unapproved vaccine, against a Virus that seems to cause more head scratching than anything...

Could they make superhumans, rid society of diseases, and eliminate their only revenue?
Could they make people with certain genetic markers infertile?

Could it be that this is only the first step of a long term plan to introduce a resistance to a much more deadly virus, in order to keep the complacent and wipe the critical thinkers? sort of creating a ROUNDUP resistant crop...

whatever this turns out to be. I will not partake in an unprecedented mass hysteria leading to unapproved mass testing, of cutting edge technology, on uninformed human beings.

a reply to: Terpene

Excellent thread OP. This technology has always peaked my interest when China I believe was on the cutting edge of it.
Kinda trippy to think about how much can be done with this. Be it good or bad.

a reply to: Allaroundyou

thank you
yes the chinese scientist making the CRISPR baby is currently in prison for it... I have a feeling this tech is bigger than we anticipate and it will have a huge impact on society... for good or bad depends mostly on our level of neophobia...

CRISPR was invented in 2012 so it has been there awhile and sometimes called as gene scissors. There have been a lot of experiments in gene editing and cloning many decades, if i remember right genes have been splitted in 1970´s. Not all is bad as there is human insulin which is also product of genetic engineering and many lives depend on it.

What really might open pandora´s box is gene hacking by biohackers and they also use CRISPR methods to do it in their own houses little labs and share their "meds" with other likeminded ppl.

a reply to: dollukka

CRISPR has not been invented, it has been discovered.

Francisco Mojica was the first researcher to characterize what is now called a CRISPR locus, reported in 1993.

.Timeline

CRISPR/CAS-9 is more than just a DNA cutter, It is going to be very interesting, to see how this turns out...

I've had the thought for many years that one day, those oh so dangerous chemistry lab sets they use to have for kids would be replaced by a genetic engineering lab kit. They could make mutant pets with different characteristics and torture them to death for amusement. Children could become angry little tyrant gods over their creations and have no value for life of any kind with that kind of "toy" to play with.

a reply to: MichiganSwampBuck

Kids will be kids😲

crRNA is different than what you see with mRNA like in the vaccines. They are incorporated as a cellular defense mechanism that goes out, tags, and breaks down its target genetic sequence. This stops protein expression, reducing virulence generation, and prevents the cell from being compromised.

Bacteria use this as an intracellular immune system because they get infected with viruses like we do. What it does is insert or removes genes that lead to protein expression. In theory it can do pretty much anything, in vitro we can change protein expression, in vivo we can do the same thing but that protein may have multiple functions and fixing or changing one aspect can have an unexpected consequence somewhere else.

This is one of the candidates that they are researching to cure HIV to go in and remove the viral genetic sequences in cells. They want to use it because HIV can incorporate differently in different cells, this will go in and grab it, removing the viral sequence from the cell or preventing it from expressing altogether. It’s pretty cool, they use a modified system to go in the cell, it identifies its target sequence in DNA, that gene is broken down and removed. The two open ends are joined together and the gene is gone or a different gene is added to that section, labeled for expression, and the cell can use the new gene.

Respiratory viruses are a little different, look up PAC-MAN, a CRISPR treatment, prophylactic or early in infection, will go in and break down viral genetic material before it can express protein. This prevents replication and infection.

a reply to: MichiganSwampBuck

you cant stop scientific progress but i share your concerns, we cant mentally and morally adapt to such a fast changing world and thats when the lack of proper education comes back biting a civilisation.

but still we dont need CRISPR for that

Children could become angry little tyrant gods over their creations and have no value for life of any kind with that kind of "toy" to play with.

some salt and a rainy day, was all that you needed to make these slugs know who was their god...

a reply to: TheAMEDDDoc

I stand corrected, and thanks for partly debunking my doomporn

Still this is enough to thread very carefull with this new technology

... in vivo we can do the same thing but that protein may have multiple functions and fixing or changing one aspect can have an unexpected consequence somewhere else...

I mean it is presumptuous to think that we can manage a mechanism that took evolution billions of years to perfect, in a couple of years.
When there are things like the "Ebbner Effect" still unexplained, I get the impression we are still mainly stumbling trough the dark, with how DNA actually works...

a reply to: Terpene

Let's not forget that the last thing to escape from Pandora's box was hope.

Gene editing if done responsibly has the potential to save millions of lives and to prevent untold suffering.

It could effectively turn thing that would kill you into things that could be treated with a simple shot.

a reply to: Terpene

Wow that is a cool post I will delve into later today, thank you.

Don’t sweat the RNA stuff, articles mix them up all the time, and I still mix them up. I remember in my first molecular biology course in grad school they showed us a couple tables that showed all the different types of RNA we need to eventually know. It looked like dozens, many were similar, and I had no idea how any human could memorize the things. Using them over and over again, it is finally making sense.

What usually gets us is bias and unintentional tunnel vision on our target. We forget that some other tissue or system uses that protein or has a similar receptor or mechanism that gets hit and we now have unintended side effects and other problems.

a reply to: AaarghZombies

still too much profit to make...

it could change everything and make several powerfull economic sectors obsolete, millions of people could loose their job...

The world would be a better place, I agree!

any volunteers?

No?

a reply to: TheAMEDDDoc

Off you go to blow some lectors head with that Info...

I`m suspecting the CRISPR Sequnces interact with the Frequencies of the magnetic field... how? why?
call it a hunch...

It's shown promise in mice models for getting rid of HIV like 40% of the time study

The other 60% turn into zombies.

a reply to: Terpene

They do not fully understand the human Genome yet, mapping it is one thing but understanding all the possible outcomes of tinkering with it they simply do not yet have that ability.

But when they do.


Cure Cystic Fibrosis, Cure Cancer, Cure Colour Blindness (though you know there are advantages and disadvantages to that since many so called colour blind people see better in dim light?).

The list goes on.

The problem is that it will turn into a designer industry and that will destroy our society and create a level of inequality's that eventually collapse of society - not it's advancement - since society is a cooperative affair, we all work toward imaginary goals that only some reach but when we know for certainty that we never shall were is the impetus to continue working toward them?.

That in turn creates rebellion.

a reply to: cooperton


can you point out how you come to the 60% zombie statement? I cant find it in the article you posted???

a reply to: LABTECH767

I remember a time where you would buy a computergame and most bugs got fixed in the devlopers house, now games are that complex that it is financially impossible to worktrough any possible scenario to fix possible bugs in house, what they did is using you the consumer as a tester, they don`t even try to fix them inhouse anymore, just release a half backed game(so called beta version) and fix the bugs as they get reported.

Now the genome is incredibly complex and working trough every possible scenario and its effects would take a long time.

So you see these DIY kits might just be that, sort of a beta version to generate more meta data to map it faster...

originally posted by: Terpene
a reply to: cooperton


can you point out how you come to the 60% zombie statement? I cant find it in the article you posted???

Hahah sorry the zombie part was a joke. But it seriously was about 40% effective getting rid of HIV. Human trials probably soon.

a reply to: cooperton

lol

good I was already doubting my reading comprehension skills