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originally posted by: HelloboysImbackguy
Disclaimer:
EVERYTHING I POST IS A LAYMAN'S VIEW. I hold no degree, have experience, am trained in this, or talk about this outside of pandemics and weirdo random conversations that dont start out about it.
I truly have no business beyond personal interest and too much time and energy to be telling anyone what is what in this subject. I read a little and like the subject is all.
Anyways:
Im thinking this has more to do with the aseptic meningitis (viral Meningitis) these people are experiencing.
The neurological component may be key. I dont think it matters anymore where the materials come from for viral replication. Better fed or sicker cells would not determine a difference in the virus pumped out either. The quantity maybe.
The type of cell would matter but this bug doesn't always look to replicate in its "preferred" cell type . It has weird triggers that go off at seemingly random intervals in each person. I dont get that.
I think the main trait that stands out is the Viruses ability to roam and search for nesting spots in the body if they dont make it to the respiratory system.
Other viruses can cause swelling of the tissue surrounding the brain. The respiratory track isnt lightyears away from the brain stem and the Medulla when it can just ride the blood stream and wait.
Our breathing and heart rate and other life sustaining involuntary reflexes are controlled here in the Medulla.
This part of the brain often swells as well with viral or bacterial meningitis. All the other symptoms match covid.
I think the area of the body where entry occurs matters as far as the viruses response to the environment.
If in the digestive system through food or liquids then diarrhea and nausea sets in and the virus wastes its novelty to our immune system by the time it gets to your body's lungs...or brain stem.
If breathed in by cough droplets, as is its preferred method, then it settles in the throat and easily works its way down into your lungs. That happens faster for some than others and that time determines the severity of the symptoms as it did before. If the body learns how to overcome it before it reaches the alveoli then you dont get it to the point that you think you are going to die.
If it makes it into the body through a skin wound or by damaged lungs or anywhere really where it is eventually flowing in our blood stream, then it starts sticking to cells once it feels its close to the brain stem. Symptoms can be worse but not have respiratory issues.
(are Ruptured Alveoli and throat abrasions the programmed entry points to the blood?)
I think it has a primary objective to get into the bloodstream in enough force to settle in the Cerebral cortex.
The primary target is not the lungs. They are used for propogation and are only a secondary component to this. I think it wants to get into the brain stem as a primary objective. Its outer layer is thick and absurdly armoured enough to roam around the body waiting to pass by the right chemical triggers to start attaching to cells for replication.
The brains is full of juicy electrical and chemical stuff.
Its like a weird combination of a more than one virus in the way it behaves.
Its ready for several environments at once.
IMO- This is connected to the ONEHEALTH cross species vaccine research.
Rift Valley Fever and a SARS type virus with the ACE2 spike already developed from one of the last epidemics?
Thats why it can infect animals we usually domesticate and us interchangeably. It is part RVF virus. The fever, confusion and problems breathing may be attributed to a neurological issue, specifically inflammation of the Medulla in the brain stem caused by a viral meningitis.
So IMO, This coronavirus is based on SARS and was combined with Rift Valley Fever virus that infects humans and livestock interchangeably and on which The Pirbright institute based its "onehealth" vaccine to be able to treat humans and animals with universal vaccines.
Possibly. I wouldn't know. Im just a conspiracy guy doing my thing.
If you do this for a living and are having a random read online. Just check it please.
Random unqualified truck driver signing off.
Cheers.
originally posted by: HelloboysImbackguy
a reply to: one4all
I dont think so personally but I think the philosophy and spiritual component of what you are saying is an interesting subject in its own right.
I just dont think it applies here. Regardless, thanks for sharing.
A replication-incompetent Rift Valley fever vaccine: Chimeric virus-like particles protect mice and rats against lethal challenge
Robert B. Mandell, Ramesh Koukuntla, [...], and Ramon Flick
Abstract
Virus-like particles (VLPs) present viral antigens in a native conformation and are effectively recognized by the immune system and therefore are considered as suitable and safe vaccine candidates against many viral diseases.
Here we demonstrate that chimeric VLPs containing Rift Valley fever virus (RVFV) glycoproteins GN and GC, nucleoprotein N and the gag protein of Moloney murine leukemia virus represent an effective vaccine candidate against Rift Valley fever, a deadly disease in humans and livestock.
RVFV is a member of the Bunyaviridae family, which includes more than 300 viruses grouped into five genera
(Orthobunyavirus, Hantavirus, Nairovirus, Phlebovirus, and Tospovirus).
Bunyaviruses are enveloped viruses with a tripartite, single-stranded RNA genome of negative and sometimes ambisense polarity
(Elliott, 1996, Elliott et al., 1991, Schmaljohn and Hooper, 2001).
These promising attempts to generate VLP-based vaccines against many different animal and human pathogens encouraged us to evaluate RVF VLPs as vaccine candidates against RVFV. Here we describe the generation of chimeric RVF VLPs, a novel concept for bunyaviruses, the optimization of VLP production and their successful use as vaccine candidates
RVFV is a prototype of emerging/re-emerging pathogens and is classified as a Category A High Priority Pathogen by the National Institute for Allergy and Infectious Diseases (NIAID)
link from study
, is on the Center for Disease Control (CDC) Bioterrorism Agents
link from study
and is also classified as a Department of Health and Human Services (HHS), United States Department of Agriculture (USDA) overlap select agent (USDA, 2005).
A formalin-inactivated RVFV vaccine, TSI-GSD-200, has been developed; however, it is not licensed and not commercially available (Pittman et al., 1999). TSI-GSD-200 is only provided to veterinarians working in endemic areas, high containment laboratory workers and others at high risk for contracting RVFV (Pittman et al., 1999). Unfortunately, th
Unfortunately, this vaccine is (i) expensive, (ii) difficult to produce, (iii) in short supply, (iv) requires larger dose relative to an attenuated vaccine and three initial inoculations followed by a 6-month booster (v) and requires continued annual boosters to maintain protective immunity (Frank-Peterside, 2000, Kark et al., 1982, Kark et al., 1985, Niklasson et al., 1985).
The use of virus-like particles (VLPs) is a promising approach for the development of a safe and efficient RVFV vaccine.
Expression of structural proteins of many non-enveloped and enveloped viruses leads to the formation of VLPs (Garcea and Gissmann, 2004, Grgacic and Anderson, 2006a, Grgacic and Anderson, 2006b, Noad and Roy, 2003).
Such VLPs frequently exhibit a morphology very similar to that of wild-type (wt) viruses (Johnson and Chiu, 2000).
Developing the vaccine
Since clinical trials are always the final stage in demonstrating that a vaccine is safe to use in humans, my colleagues and I started by using a technique that has extensively been shown to be safe for human vaccination against a host of diseases, including influenza, malaria, and Ebola.
The technique uses a chimpanzee respiratory (adenovirus) virus to expose individuals to just a small part of the Rift Valley Fever virus – enough for the immune system to recognise a future infection, but not enough to cause an infection in itself.
As this incomplete virus is markedly different to the full virus, the technique allows animal health practitioners to accurately test whether an animal is vaccinated, or an infected but symptomless carrier.
This is crucial during outbreaks, as false positives can lead to unnecessary culling of livestock, increased costs, and reduced cooperation of farmers in the fight to control the disease
Rapid development of vaccines against emerging pathogens: The replication-deficient simian adenovirus platform technology
Adenoviruses can survive on plastic and metal surfaces — think countertops and hospital tables— for a month. Some formulations of alcohol and chlorhexidine do not kill them easily, tests have shown, although chlorine does. That makes cleanup tricky after an outbreak.
I wonder if they find any similarity in the genetic codes of each.
originally posted by: HelloboysImbackguy
a reply to: one4all
I see what you are saying as far as how bacteria would behave differently depending on where they call home in the body
I am still interested in discussing the effects this virus has to our digestive system and if it is interacting with the bacterias in such a way as to modify or infect them.
Then, Is this localized infection of the digestive bacterias producing a virus with any noticeable difference to those produced from human cells? That would be worth knowing.
I am also every interested in how polymorphic bacteria could be linked to that process of digestive bacterial infection by Covid 19.
I didnt want to come off as dismissive. I have a limited knowledge of EM and bio-electric resonance and biological functions associated with it. I thought that was the ultimate path you wanted to lead to.
Even if it is, why not?! Lol forgive me for being presumptuous.
I was a waiter, barman, liquor retailer, butcher, owned a butcher shop in NYC for a while, promotional field coordinator for liquor tastings, owned a cafe in northern Spain for a while, was regional coordinator and team leader doing canvassing and petition work in multiple states during the last presidential election, lifeguard, um, garbage man for a day, was a licensed insurance agent in NY, and other stuff I cant remember. /end resume over. Lol.
Im just a driver and job foreman for a NYC based moving company now. I wasn't doing long distance trips anymore and wanted to be close to the family.
I dont have any relevant qualifications in this subject but I know how things work.
Its also something to do that reduces anxiety by getting more information about our current situation. "Know what you are facing" sort of thing. I am not as worried as I was, but I am concerned that they are still only looking at this virus like its a respiratory disease while ignoring the neurological aspect of the infections.
Have a good one
Hold it down.
Upon coexpression of M, S, and E by using the vaccinia virus T7 system (20), virus-like particles (VLPs) containing these three viral membrane proteins were assembled in and released from cells.
However, only M and E were required for particle formation. The S protein was dispensable but was incorporated when present (6, 73).
The envelope particles produced by this system were shown to form a homogeneous population of spherical particles indistinguishable from authentic virions in size and shape (73).
One of our main interests is to understand the process of coronavirus assembly.
We are particularly interested in the interactions and the mechanisms that drive the formation of the viral particles. With the VLP assembly system, we have developed an ideal tool for the study of coronavirus envelope formation and for the analysis of the interactions between the viral membrane proteins in molecular detail
originally posted by: HelloboysImbackguy
a reply to: HelloboysImbackguy
Related to: Virus like particles and artificially created coronavirus lipid protective layer that can be programmed to hold Viral RNA
Upon coexpression of M, S, and E by using the vaccinia virus T7 system (20), virus-like particles (VLPs) containing these three viral membrane proteins were assembled in and released from cells.
However, only M and E were required for particle formation. The S protein was dispensable but was incorporated when present (6, 73).
The envelope particles produced by this system were shown to form a homogeneous population of spherical particles indistinguishable from authentic virions in size and shape (73).
One of our main interests is to understand the process of coronavirus assembly.
We are particularly interested in the interactions and the mechanisms that drive the formation of the viral particles. With the VLP assembly system, we have developed an ideal tool for the study of coronavirus envelope formation and for the analysis of the interactions between the viral membrane proteins in molecular detail
www.ncbi.nlm.nih.gov...
This study shows that the ability to make a VLP with a coronavirus membrane is possible and already used in vaccine research /treatment.
In this study they essentially built a dummy " shell " coronavirus particle and programmed its self replication...so it could make more shells....which COULD be filled with the genetic fragments of Viruses you want to vaccinate against in a seperate process...
These " Virus Like Particles " are indistinguishable from actual viruses. Their morphology is identical to wild type viruses.
I will keep adding more, but I think Im right. And Im the only one saying this about Covid-19...and that is worrying me.
A lot.