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Taciana Sherlock from the Brazilian wildlife control agency Ibama said the toucan was rescued in March, malnourished and missing its beak.
Black-beaked toucans like Tieta, which are native to Rio and not endangered, can cost up to $5,000 (£3,180) when sold legally, according to Ms Sherlock.
She says that before the surgery Tieta was using the lower part of the beak to throw food into the air and trying to grab it.
She only succeeded once in every three attempts.
"It took her three days to realise she had it again," says Instituto Vida Livre Director Roched Seba.
"We were feeding her fruit and she was ignoring the new beak. But when we gave her live animals, like maggots and cockroaches, she ate normally immediately," he explains.
"I believe she had that kind of food when she was free, before losing the beak. So it activated a core memory," he adds.
It took researchers three months to design the beak but it took only two hours for the printer to print it. - the beak weighs approximately 4g and it is 4cm (1.6in) long.
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