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originally posted by: JustMike
a reply to: jadedANDcynical
Thanks for posting.
Just making sure I read that right: the relative is a nurse, but is also a patient?
Oh, and please check your messages. Thanks.
originally posted by: jadedANDcynical
All I want is for everyone in the world to respect this virus for what it has shown us it can do.
It is this person's opinion that the virus is, and has been, transmissible prior to onset of symptoms.
That is the bigges lie we are being told.
originally posted by: ~Lucidity
a reply to: jadedANDcynical
Add another item to the things they-don't-know or we-aren't-being-told list: That is may be transmissible after "cure" or "recovery."
And I still think Duncan was a cover-up for someone (read that as someone who was brought here intentionally with it) spreading the disease.
Ebola virus (EBOV, formerly designated Zaire ebolavirus) is the most dangerous of the five known viruses within the genusEbolavirus.[1]
Abstract
Filoviruses are enveloped, nonsegmented negative-stranded RNA viruses. The two species, Marburg and Ebola virus, are serologically, biochemically, and genetically distinct. Marburg virus was first isolated during an outbreak in Europe in 1967, and Ebola virus emerged in 1976 as the causative agent of two simultaneous outbreaks in southern Sudan and northern Zaire. Although the main route of infection is known to be person-to-person transmission by intimate contact, the natural reservoir for filoviruses still remains a mystery.
Abstract
The Ebola filoviruses are aggressive pathogens that cause severe and often lethal hemorrhagic fever syndromes in humans and nonhuman primates. To date, no effective therapies have been identified. To analyze the entry and fusion properties of Ebola virus, we adapted a human immunodeficiency virus type 1 (HIV-1) virion-based fusion assay by substituting Ebola virus glycoprotein (GP) for the HIV-1 envelope. Fusion was detected by cleavage of the fluorogenic substrate CCF2 by beta-lactamase-Vpr incorporated into virions and released as a result of virion fusion. Entry and fusion induced by the Ebola virus GP occurred with much slower kinetics than with vesicular stomatitis virus G protein (VSV-G) and were blocked by depletion of membrane cholesterol and by inhibition of vesicular acidification with bafilomycin A1. These properties confirmed earlier studies and validated the assay for exploring other properties of Ebola virus GP-mediated entry and fusion. Entry and fusion of Ebola virus GP pseudotypes, but not VSV-G or HIV-1 Env pseudotypes, were impaired in the presence of the microtubule-disrupting agent nocodazole but were enhanced in the presence of the microtubule-stabilizing agent paclitaxel (Taxol). Agents that impaired microfilament function, including cytochalasin B, cytochalasin D, latrunculin A, and jasplakinolide, also inhibited Ebola virus GP-mediated entry and fusion. Together, these findings suggest that both microtubules and microfilaments may play a role in the effective trafficking of vesicles containing Ebola virions from the cell surface to the appropriate acidified vesicular compartment where fusion occurs. In terms of Ebola virus GP-mediated entry and fusion to various target cells, primary macrophages proved highly sensitive, while monocytes from the same donors displayed greatly reduced levels of entry and fusion. We further observed that tumor necrosis factor alpha, which is released by Ebola virus-infected monocytes/macrophages, enhanced Ebola virus GP-mediated entry and fusion to human umbilical vein endothelial cells. Thus, Ebola virus infection of one target cell may induce biological changes that facilitate infection of secondary target cells that play a key role in filovirus pathogenesis. Finally, these studies indicate that pseudotyping in the HIV-1 virion-based fusion assay may be a valuable approach to the study of entry and fusion properties mediated through the envelopes of other viral pathogens.
originally posted by: ~Lucidity
a reply to: jadedANDcynical
It is this person's opinion that the virus is, and has been, transmissible prior to onset of symptoms.
That is the bigges lie we are being told.
Add another item to the things they-don't-know or we-aren't-being-told list: That is may be transmissible after "cure" or "recovery."
Ok I am here, now that we have come close to the end of the other thread, I was looking for a new ongoing home to share information on
this global crisis.
Read where soficrow had suggested to someone one of your ongoing threads.
originally posted by: soficrow
a reply to: jadedANDcynical
Thanks for doing this.
Am hoping other people might have inside sources too and also post their info here.