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Molecular Diagnostics for Lassa Fever at Irrua Specialist Teaching Hospital, Nigeria: Lessons Learnt from Two Years of Laboratory Operation
……..The study was classified as a service evaluation and granted exemption from ethical review by the Research and Ethics Committee of ISTH. …….Service evaluation is exempt from ethical review according to the National Code of Health Research Ethics, National Health Research Ethics Committee, Federal Ministry of Health, Nigeria.
About the Viral Hemorrhagic Fever Consortium
The Viral Hemorrhagic Fever Consortium was established in 2010 as a result of several multi-year grants and contracts awarded to Tulane University by the National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Health (NIH), …..The Consortium is a collaboration between …..the Kenema Government Hospital (Sierra Leone), the Irrua Specialist Teaching Hospital (Nigeria) and various other partners in West Africa. More information teachingis available at www.vhfc.org .
Liberia Declares State of Emergency as Ebola Toll Rises
State of emergency declared in Liberia
The state of emergency allows Liberia’s government to curtail civil rights and to deploy troops and police to impose quarantines on badly affected communities to contain an epidemic that has struck four west African nations.
Liberia, Sierra Leone Race To Enforce Ebola Quarantine
….Underscoring desperate attempts to stop the disease, troops in full combat gear deployed in the rain to block people traveling to Liberia's capital from rural areas hit by Ebola. Liberian President Ellen Johnson Sirleaf declared a national state of emergency, and officials said Thursday that no one with a fever would be allowed in or out of the country.
In Sierra Leone, military forces also deployed as part of "Operation Octopus" which officials said was aimed at preventing "the unauthorized movement of Ebola-infected persons."
…Experts warned that extreme measures risk driving patients and their families further underground.
….Sirleaf justified the 90-day state of emergency ….She warned that some civil liberties could be suspended. By Thursday, soldiers were already restricting movements on the roads to Monrovia, witnesses said. Some soldiers were deployed to the crossroads town of Klay, about 25 miles (40 kilometers) west of Monrovia in an effort to stop people from three Ebola-infected counties from coming closer to the capital.
The capital already has been hit by the virus, with bodies abandoned in the streets. Relatives are hiding feverish patients at home for fear if they are brought to isolation centers and don't have Ebola, they will end up contracting it anyway.
National Health Workers Association President Joseph Tamba said the state of emergency is necessary. But he says people should have been given advance notice to buy food ahead of the movement restrictions.
The Centers for Disease Control and Prevention (CDC) has raised their emergency response to the Ebola outbreak to level 1.
This is the highest level of emergency response at the CDC and, according to an agency spokesperson, it has been activated because of the surge of personnel being sent into the affected countries.
…According to the CDC, level 1 is “all hands on deck.” The CDC has only been to level 1 three times in the history of the emergency operations center, which opened in 2003. Previously, it was activated for Hurricane Katrina in 2005 and the H1N1 influenza outbreak in 2009.
No one can leave Kenema
Ebola Frontline: Sierra Leone’s Ebola Epicenter is On Lockdown
When President Ernest Bai Koroma announced a State of Emergency on July 30, many Sierra Leonians hoped that it would mean the government would finally get a handle on the unprecedented Ebola outbreak. However, no one knew until early this morning that for some, this state of emergency meant they were trapped in the epicentre of this epidemic.
….All districts of Sierra Leone except one have recorded cases of Ebola, but the hardest hit are the districts of Kenema and Kailahun. Kenema is the third largest city in Sierra Leone, and the centre of the country’s lucrative diamond trade, while Kailahun is a major regional hub. The first recorded case in Sierra Leone was confirmed in Kailahun, and to date, there have there have been 570 confirmed Ebola cases in these two districts alone. This is epicenter of Ebola in Sierra Leone, and as of this morning, the districts of Kenema and Kailahun, with a combined population of over 950,000, are effectively locked down. Blockades on the major highways are ensuring that no one leaves or enters.
…“No one can leave Kenema,”…
…the ramifications are already being felt. The price of rice, an essential food in Sierra Leone, has already increased by 30 percent today alone. In the central market, the price of salt has doubled in the last 24 hours. …
originally posted by: soficrow
Sawyer is being blamed for bringing Ebola to Nigeria but I call bs - it started in Nigeria's Irrua Specialist Teaching Hospital with the 'research' arm.
originally posted by: loam
.....What does Nigeria's Irrua Specialist Teaching Hospital have to do with the outbreak in Nigeria?
I'm in several ebola threads, maybe I missed something?
Molecular Diagnostics for Lassa Fever at Irrua Specialist Teaching Hospital, Nigeria: Lessons Learnt from Two Years of Laboratory Operation
…….Only the laboratory at the hospital in Kenema, Sierra Leone, which has become operational since 2004 (after civil war forced its closure in 1993), is able to perform Lassa fever testing for patients [30]. In Nigeria, the situation improved with the implementation of Lassa virus PCR testing at a research laboratory of the University of Lagos ….
…….The diagnostic and research laboratory was built in 2008 and started operation in September 2008.
……..The study was classified as a service evaluation and granted exemption from ethical review by the Research and Ethics Committee of ISTH. …….Service evaluation is exempt from ethical review according to the National Code of Health Research Ethics, National Health Research Ethics Committee, Federal Ministry of Health, Nigeria.
Guinea shuts borders with Sierra Leone and Liberia
Guinea announced the closure of its borders with Sierra Leone and Liberia on Saturday in a bid to halt the spread of Ebola, a virus that has killed nearly 1,000 people in the three countries this year.
….Nigeria became the third African nation, after Sierra Leone and Liberia, to declare a national emergency on Friday as the region's healthcare systems struggle to cope with the advance of one of the deadliest diseases known to man.
Nigeria declares state of EMERGENCY as Guinea CLOSES borders
For this reason, spread within Africa is really what public health officials are worried about. “Our first concern is that this is going to go into adjacent areas through people traveling in the region,” said Daniel Bausch, associate professor at the Tulane University School of Public Health and Tropical Medicine, who is working with the WHO and MSF on the outbreak. “In the short term, the main vector is the traveler: local people traveling from one village to the next, on more regional scale, plane travelers.” This pie chart shows the final destinations of travelers originating in the three countries currently most affected by Ebola. As you can see, travel from Sierra Leone, Guinea and Liberia within the continent is much more prevalent than travel elsewhere. All countries in West Africa are already on alert. National authorities in Ghana, Nigeria, Togo and the Côte d’Ivoire are working with the WHO on prevention efforts and monitoring potential cases. To do this, contact tracing is essential, said Bausch.
...“With Ebola outbreaks, most of the time there’s one or very few introductions of the virus from the wild into humans, and all the transmission after that is human-to-human transmission. So people who are traveling locally as well as on planes and other modes of transport, that’s the way this would get around.”
....“The worst-case scenario is that the disease will continue to bubble on, like a persistent bushfire, never quite doused out,” said Derek Gatherer, a Lancaster University bio-informatician who has studied the evolution of this Ebola outbreak. “It may start to approach endemic status in some of the worst affected regions. This would have very debilitating effects on the economies of the affected countries and West Africa in general.”
This dire situation could come about because of a “persistent failure of current efforts,” he added. “Previous successful eradications of Ebola outbreaks have been via swamping the areas with medical staff and essentially cutting the transmission chains. Doing that here is going to be very difficult and expensive. We have little option other than to pump in resources and engage with the problem using the tried-and-tested strategy—but on a scale previously unused.”
This approach indicates that the outbreak in Guinea is likely caused by a Zaire ebolavirus lineage that has spread from Central Africa into Guinea and West Africa in recent decades, and does not represent the emergence of a divergent and endemic virus.
As the GP sequences show, without more diverse sequences, especially those from the animal reservoir, it is difficult to narrow down the estimates of when and through what means the Central African EBOV lineage has been introduced into West Africa.
The branch leading to the Guinea outbreak is long, not because it is a divergent lineage but because it is the most recently sampled so has had the most time to evolve.
originally posted by: jadedANDcynical
a reply to: soficrow
Maybe not a new subclade, but an evolved form of the older ZEBOV:
Novel Zaire Ebola Sub-Clade In Guinea and Sierra Leone
All 101 sequences form a Zaire sub-clade which signals clonal expansion due to human to human transmission following a single introduction. These 101 sequences are easily distinguished from all other Zaire sub-clades, which have been associated with the largest number of reported Ebola outbreaks, including the previously most deadly outbreak in Yambuku, Zaire in 1976 (280 deaths in 318 cases), which was followed by the second most deadly outbreak, which was 19 years later in Kikwit, Zaire in 1995 (245 deaths in 317cases).
Full-length genome sequencing and phylogenetic analysis showed that EBOV from Guinea forms a separate clade in relationship to the known EBOV strains from the Democratic Republic of Congo and Gabon.
1) quarantine has been historically used to discriminate against minorities;
2) studies demonstrate that mass quarantine is ineffective;
3) a large scale quarantine would be difficult to implement.
An outbreak should meet the following three criteria for quarantine to be a useful measure of disease control:
* people likely to be incubating the infection must be efficiently and effectively identified;
* those people must comply with the conditions of quarantine; and
* the infectious disease in question must be transmissible in its presymptomatic or early symptomatic stages.
The use of quarantine in the Toronto (SARS) outbreak failed on all three counts.
Ebola virus: Liberia health system 'falling apart'
….The MSF co-ordinator in Liberia said official figures were "under-representing the reality", and that the health system was "falling apart".
…Meanwhile, neighbouring Guinea has denied earlier reports that it had sealed its borders.
…In Guinea, the health minister on Saturday said the borders with Liberia and Sierra Leone had been closed to prevent infected people crossing into the country.
However, state television later said: "Guinea has not closed its borders with Sierra Leone or with Liberia. It's rather that we have taken health measures at the border posts."
A government source told Reuters that the minister who made the original announcement had not been in possession of accurate information.
Nigeria Struggles to Cope With Ebola Outbreak
Nigerian health officials have announced 10 confirmed cases and two deaths in the country from the Ebola outbreak that is sweeping West Africa, including a nurse and a man from Liberia whom the nurse had been caring for.
…“Rapid epidemic transmission has been with us a long time, but my guess is that it’s accelerating, with the number of people on the move and intensity of air travel, global trade and the numbers of displaced people we have globally,” said Jeffrey D. Sachs, an economist and the director of the Earth Institute at Columbia University.
Climate change, population growth and an increase in displaced populations mean that people are pushing into previously uninhabited places, creating new vulnerabilities and bringing humans into closer contact with animal populations, where many of the diseases have begun, he said.
….At the same time, globalization means that people are mixing more, trading more and handling more farm animals in industrial settings — all of which facilitate the emergence and spread of infectious diseases.
“This ought to force a reflection,” Mr. Sachs said, adding that establishing a basic network of community health workers across the developing world was an urgent priority.
One of those Nigerian health care workers, Obi Justina Ejelonu, who was on duty at the First Consultant Hospital in Obalende, Lagos when Sawyer was brought in, is said to be clinging onto her life after contracting the virus. Ironically, Obi Justina had initially posted a message on her Facebook page boasting that she had limited contact with Sawyer and was grateful to God to be alive:
Obi Justina wrote: “I never contacted his fluids. I checked his vitals, helped him with his food (he was too weak)…..I basically touched where his hands touched and that’s the only contact. Not directly with his fluids. At a stage, he yanked off his infusion and we had blood everywhere on his bed…..but the ward maids took care of that and changed his linens with great precaution.”
Link.
Isn't that just the simple explanation of what a subclade is?
We believe that at present no suitable outgroup sequences to root the EBOV phylogeny exist and that a temporal rooting gives the most consistent results.
This approach indicates that the outbreak in Guinea is likely caused by a Zaire ebolavirus lineage that has spread from Central Africa into Guinea and West Africa in recent decades, and does not represent the emergence of a divergent and endemic virus.
As the GP sequences show, without more diverse sequences, especially those from the animal reservoir, it is difficult to narrow down the estimates of when and through what means the Central African EBOV lineage has been introduced into West Africa.