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Niemiller and his colleagues decided to try a different approach, called eDNA. They sampled water in seepage springs where S. hayi and other Stygobromus species had previously been sighted and in others where they had not. The team filtered the water on site, returning to the lab to check their filters for the animal's DNA.
"This environmental DNA approach has successfully detected larger aquatic animals like salamanders, but no studies had previously used this technique to hunt for invertebrates in groundwater," Niemiller said.